Plasma 1x (Human-Tech) DNA Free 5 ml

Plasma 1x (human-tech) DNA free is a groundbreaking product designed for researchers and laboratories seeking high-quality human plasma without the presence of DNA. This innovative solution mimics the properties of human plasma obtained from clinical blood draws and subsequent plasma separation. It provides an essential resource for various scientific applications, including molecular biology and genetic research.

This product contains all the vital components found in human plasma, such as human proteins in standard concentrations, electrolytes, and EDTA (ethylenediaminetetraacetic acid). The formulation adheres strictly to the guidelines set forth by the International Council Society of Hematology (ICSH) and the Clinical and Laboratory Standards Institute (CLSI) regarding EDTA levels in blood collection tubes. Such adherence to standards guarantees the reliability and accuracy of your research outcomes.

Key Ingredients and Composition

• Human Proteins: Each batch of Plasma 1x contains approximately 50 mg/ml of human proteins. These proteins are crucial for various biochemical processes and studies, providing researchers with a relevant matrix for their experiments.
• Electrolytes: The product is enriched with essential electrolytes, including sodium (Na+), potassium (K+), calcium (Ca2+), chloride (Cl–), and phosphate (PO43-). These electrolytes are vital for maintaining the physiological balance and functionality of cells, making them important for any experiments involving cellular biology or biochemistry.
• EDTA Concentration: The plasma contains EDTA at a concentration of 1.42 mg/ml. EDTA is a well-known chelating agent that prevents coagulation and maintains sample integrity during experiments.

Recommended Uses

Plasma 1x (human-tech) DNA free is particularly useful for two main applications:

1. As a No Template Control (NTC): In molecular experiments, it’s essential to include controls to ensure the accuracy of your results. Plasma 1x serves as an effective NTC, helping to validate that no extraneous DNA is affecting the experimental outcomes.
2. For Spike-in Experiments: This product is ideal for spike-in experiments involving individual circulating free DNA (cfDNA) and circulating tumor DNA (ctDNA). Researchers can use Plasma 1x to introduce known quantities of these DNA types into their studies, enhancing the precision and reliability of their results.

Quality Control and Testing

To ensure the highest quality, Plasma 1x undergoes rigorous testing. This includes:

• DNA Quantification: The product is tested for any residual human DNA, following ISO17025 standards. This step is crucial for maintaining the integrity of the research.
• Fragment Analysis: Utilizing the High Sensitivity DNA Kit from Agilent, the product undergoes fragment analysis to ensure it meets the required specifications.