Ashkenazim Son FFPE Reference Standard Curl: High-Quality Control for Genomic Assays

The Ashkenazim Son FFPE Reference Standard Curl is a top-tier commutable FFPE control specifically designed to replicate patient tissue samples in laboratory settings. This product is derived from cell lines and serves as an ideal full workflow control for diagnostic assays, suitable for both clinical laboratories and R&D departments. Optimized for use as a wildtype control for most cancer-related genes, this FFPE reference material ensures high accuracy and reliability in clinical genomics workflows.

The Ashkenazim Son FFPE Reference Standard Curl is essential for a variety of applications:

• Validation and Development: Perfect for refining and validating DNA and RNA extraction protocols, ensuring robust sequencing and PCR workflows.
• Sequencing Protocol Control: Use for Whole Genome Sequencing (WGS), Whole Exome Sequencing (WES), Amplicon Sequencing, and optimizing PCR protocols.
• Detection Limit Assessment: Accurately determine the sensitivity of your methods for reliable results.
• Calibration and Workflow Development: Ideal for instrument calibration and development in DNA processing techniques such as acoustic shearing, enzymatic digestion, or sonication.
• NGS Pipeline Performance: Benchmark your Next Generation Sequencing (NGS) pipeline against freely available datasets to ensure consistent performance.
• Routine Monitoring: Suitable for ongoing performance monitoring (Research Use Only) to maintain high-quality standards.

Product Overview

• Format: Formalin Fixed Paraffin Embedded (FFPE)
• Unit Size: 5 FFPE curls per vial, with each curl being 10 µm thick
• DNA Yield: ≥ 400 ng (using Qiagen AllPrep DNA/RNA FFPE kit)
• RNA Yield: ≥ 400 ng (using Qiagen AllPrep DNA/RNA FFPE kit)
• Mutations: Wildtype for most cancer-relevant mutations, genome thoroughly sequenced and tested for specific mutations using ddPCR
• Allelic Frequency: None
• Storage: 2-8 °C, stable for 24 months from manufacturing
• Quality Control:
  • DNA: ddPCR for allelic frequency, Qubit for DNA quantification, agarose gel electrophoresis for quality assessment
  • RNA: Fluorometric measurement using Qubit
  • Other: Cell density verified via microscopy and calibrated measurements

The Ashkenazim Son FFPE Reference Standard Curl delivers unmatched consistency and reliability, making it a must-have for any lab focused on high-precision genomic research.