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5FU DPYD Stripassay

Short description

The Viennalab PGX-5FU DPYD XL StripAssay® is a comprehensive solution for accurate identification of the four most clinically relevant variants in the dihydropyrimidine dehydrogenase (DPYD) gene with therapeutic relevance for fluoropyrimidine treatment. The kit is designed to increase patient safety and reduce the risk of treatment-related toxicity in cancer therapy.

Fluoropyrimidines are widely used in cancer therapy, but some patients may develop severe treatment-related toxicity due to genetic variants in the DPYD gene, impairing the activity of dihydropyrimidine dehydrogenase (DPD), a key metabolic enzyme. The kit supports DPYD genotype-guided fluoropyrimidine dosing recommended by the European Medicines Agency to personalize the fluoropyrimidine dosage and improve patient outcomes.

Product highlights

  • Fluoropyrimidines (5-Fluorouracil, Capecitabine, and Tegafur) are widely used
  • Some patients may experience severe treatment toxicity
  • This impaired activity is caused by genetic variants in the DPYD gene
  • The CPIC Guidelines recommend DPYD genotype-guided fluoropyrimidine dosing

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5FU DPYD Stripassay

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Instructions for Use

Brochure

CPIC Guideline

MSDS

 

Specifications of the 5FU DPYD Stripassay

Introducing the Viennalab PGX-5FU DPYD XL StripAssay® – the comprehensive solution for accurate identification of the four most clinically relevant variants in the dihydropyrimidine dehydrogenase (DPYD) gene, with therapeutic relevance for fluoropyrimidine treatment. Our kit is designed to increase patient safety and reduce the risk of treatment-related toxicity in cancer therapy.

Fluoropyrimidines, including 5-Fluorouracil, Capecitabine, and Tegafur, are widely used in cancer therapy, but some patients may develop severe treatment-related toxicity. The Viennalab PGX-5FU XL StripAssay® covers the four most clinically relevant variants in the DPYD gene that can impair the activity of dihydropyrimidine dehydrogenase (DPD), a key metabolic enzyme for fluoropyrimidine treatment.

The European Medicines Agency recommends DPYD genotype-guided fluoropyrimidine dosing to increase patient safety, and the Viennalab PGX-5FU XL StripAssay® is designed to support these guidelines. By accurately identifying the patient’s DPYD genotype, clinicians can personalize the fluoropyrimidine dosage to individual patient needs, reducing the risk of treatment-related toxicity and improving patient outcomes.

Trust in Viennalab’s expertise and experience in molecular diagnostics to provide you with reliable and easy-to-use results for your laboratory workflow. Take the first step towards achieving personalized fluoropyrimidine-based therapy and improving patient outcomes – add the Viennalab PGX-5FU XL StripAssay® to your laboratory today.

Components of the 5FU DPYD Stripassay
  1. DNA Extraction Kit: A kit containing all the reagents necessary for the extraction of genomic DNA from a blood or saliva sample, including DNA extraction buffer, proteinase K, and magnetic beads.
  2. StripAssay: A test strip containing immobilized probes specific for the detection of genetic polymorphisms in the DPYD gene, including alleles *2A, *2B, *3, and *13.
  3. Conjugate: A solution containing probes labeled with biotin that bind to the immobilized probes on the StripAssay.
  4. Enzyme Conjugate: A solution containing streptavidin conjugated to horseradish peroxidase (HRP) that binds to the biotin-labeled probes on the StripAssay.
  5. Substrate: A solution containing a chromogenic substrate for HRP that produces a color reaction upon cleavage by the enzyme.
  6. Stop Solution: A solution used to stop the enzymatic reaction and stabilize the color reaction.
  7. Control DNA: A synthetic DNA that is added to the sample as an internal control to ensure that the assay is working correctly.
  8. DNase/RNase-free Water: Water that has been treated to remove contaminating DNases and RNases, which can degrade the extracted DNA.

 

The kit also includes instructions for use, as well as a protocol for the detection of genetic polymorphisms in the DPYD gene using the StripAssay format.

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