BIOSYNEX AMPLIQUICK® Fecal Pretreatment is a manual kit designed to screen for intestinal parasites through gene amplification. It comes with pre-filled vials containing ready-to-use grinding beads and pretreatment buffer to release nucleic acids from biological samples. The kit is intended to be used by laboratory staff for in vitro molecular diagnostics only and is validated for use with BIOSYNEX AMPLIQUICK® Helminths and Protozoans PCR kits. The kit optimizes the release of DNA contained in biological samples to perform molecular detection testing, which helps identify both silent and acute infections. The kit uses a physico-chemical lysis method, involving mechanical grinding and heating to 95°C to increase the ratio of non-human DNA extracted.

The pretreatment medium is specifically designed to allow DNA to separate from impurities, PCR inhibitors, and enzymes present in large quantities in feces. After centrifugation, the DNA remains suspended in the supernatant, while the sample matrix is eliminated in the pellet. This kit is an essential tool for in vitro molecular diagnostics, providing accurate and sensitive detection of intestinal parasites in fecal samples.