Hot MolTaq 16S/18S Polymerase is a highly purified thermostable enzyme that is isolated from Thermus aquaticus. It is specifically designed for PCR amplification of DNA from challenging samples. This enzyme has a high specific activity and a high processivity, which enables it to amplify DNA fragments up to 5 kb in length. The enzyme has a proofreading activity, which makes it highly accurate in the replication of DNA. Additionally, Hot MolTaq 16S/18S Polymerase has a low error rate, which means that it is highly reliable in PCR experiments.

Hot MolTaq 16S/18S Polymerase is engineered to have an optimized buffer system, which is designed to perform under challenging PCR conditions. The enzyme can perform well in the presence of inhibitors such as salts, detergents, and complex biological fluids. Hot MolTaq 16S/18S Polymerase is also designed to perform under fast PCR conditions, providing rapid and efficient amplification of DNA in a short time.

Advantages:

There are several advantages of using Hot MolTaq 16S/18S Polymerase in PCR experiments. Firstly, the enzyme has a high sensitivity, which means that it can amplify low amounts of DNA from challenging samples. This is particularly useful in experiments where the starting material is limited or contains inhibitors that can interfere with PCR amplification. Secondly, Hot MolTaq 16S/18S Polymerase has a high yield, which means that it can generate a large amount of amplified DNA in a short period of time, even under challenging PCR conditions.

Another advantage of Hot MolTaq 16S/18S Polymerase is its versatility in PCR amplification of DNA from a variety of sources. The enzyme can amplify DNA from different organisms, tissues, and fluids, making it a valuable tool for molecular biology experiments. Additionally, Hot MolTaq 16S/18S Polymerase is compatible with a variety of PCR buffers and conditions, which makes it easy to use in different experimental setups.

Applications:

Hot MolTaq 16S/18S Polymerase has several applications in molecular biology research. It is commonly used in PCR experiments for the amplification of DNA fragments for sequencing or cloning. The enzyme is also used in the detection of microbial pathogens in clinical and environmental samples. Hot MolTaq 16S/18S Polymerase is particularly useful in the amplification of 16S and 18S rRNA genes, which are commonly used as genetic markers for microbial identification and taxonomy.