Plasma 1x (Human-Tech) DNA Free 40 ml

Plasma 1x (human-tech) DNA free 40 ml is a state-of-the-art solution designed specifically for research applications that require human plasma without any DNA contamination. This product is expertly recreated to closely mimic the composition of human plasma obtained through clinical blood draws and plasma separation processes. It serves as a crucial resource for scientists and researchers engaged in molecular biology, genomics, and various other fields where the purity of samples is paramount.

This innovative plasma formulation includes all essential components typically found in human plasma. Each 40 ml bottle contains standard concentrations of human proteins, necessary electrolytes, and the chelating agent EDTA (ethylenediaminetetraacetic acid). The concentration of EDTA in Plasma 1x complies with guidelines set by the International Council Society of Hematology (ICSH) and the Clinical and Laboratory Standards Institute (CLSI), ensuring that it meets the highest quality and safety standards for use in blood collection tubes.

Key Components

• Human Proteins: Plasma 1x contains approximately 50 mg/ml of human proteins, essential for numerous biochemical reactions and cellular functions. These proteins provide a relevant matrix for a variety of research applications.
• Electrolytes: This plasma solution is fortified with crucial electrolytes, including sodium (Na+), potassium (K+), calcium (Ca2+), chloride (Cl–), and phosphate (PO43-). These electrolytes play a vital role in maintaining cellular homeostasis and support various experimental processes.
• EDTA Concentration: The product contains EDTA at a concentration of 1.42 mg/ml, acting as an effective anticoagulant to prevent clotting and maintain sample integrity during experimentation.

Recommended Applications

Plasma 1x (human-tech) DNA free 40 ml is versatile and can be employed for various purposes, including:

1. As a No Template Control (NTC): In molecular experiments, including PCR (Polymerase Chain Reaction), having a reliable NTC is critical. Plasma 1x serves as an ideal control, ensuring that any detected DNA is due to the experimental variables and not from extraneous sources.
2. For Spike-in Experiments: This product is particularly beneficial for spike-in experiments with circulating free DNA (cfDNA) and circulating tumor DNA (ctDNA). Researchers can introduce known quantities of cfDNA or ctDNA into their samples to evaluate their effects on analytical results, improving the accuracy of their findings.

Storage and Stability

To ensure optimal performance, Plasma 1x should be stored at temperatures between 2-8 °C. When stored correctly, the product maintains its stability for up to 24 months from the date of manufacture. This longevity provides researchers with a reliable product for long-term studies and projects.

Quality Assurance

Quality control is a top priority for Plasma 1x. The product undergoes extensive testing to ensure it meets stringent quality standards:

• DNA Quantification: Each batch is rigorously tested for residual human DNA, adhering to ISO17025 standards to confirm the absence of contamination.
• Fragment Analysis: The product is also subjected to fragment analysis using the High Sensitivity DNA Kit from Agilent. This step ensures that the plasma meets required specifications for purity and quality.

In summary, Plasma 1x (human-tech) DNA free 40 ml is an invaluable resource for researchers and laboratories requiring high-quality, DNA-free human plasma. Its precise formulation, adherence to quality standards, and versatility make it a preferred choice for a wide range of scientific applications.