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TPMT Stripassay

Short description

The Viennalab TPMT StripAssay® Kit is a reliable solution for accurate identification of genetic variants affecting the metabolism of thiopurine-based therapeutic drugs. The kit identifies TPMT variants of therapeutic relevance, enabling clinicians to personalize the thiopurine dosage to individual patient needs.

Patients carrying certain TPMT genetic variants are at risk of thiopurine toxicity, which can lead to life-threatening conditions. By accurately identifying the patient’s TPMT isoform, clinicians can avoid toxic effects and improve patient outcomes.

Product highlights

  • For identifying genetic variants  of thiopurine-based drugs
  • Identifies TPMT variants of therapeutic relevance
  • Certain TPMT genetic variants are at risk of thiopurine toxicity
  • Helps clinicians avoid toxic effects

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TPMT Stripassay

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Specifications of the TPMT Stripassay

Introducing the Viennalab TPMT StripAssay® Kit – the crucial solution for accurate identification of the most frequent TPMT variants affecting the metabolism of thiopurine-based therapeutic drugs. Our kit enables clinicians to identify TPMT variants of therapeutic relevance, enabling personalized treatment to individual patient needs.

Thiopurines are commonly used in the treatment of leukemias and autoimmune disorders, as well as in immunosuppression therapy after organ transplantation. However, patients carrying certain genetic variants of the drug-metabolizing enzyme TPMT are at risk of thiopurine toxicity due to the accumulation of the drug to toxic levels.

The Viennalab TPMT StripAssay® Kit identifies the most frequent TPMT variants of therapeutic relevance, enabling clinicians to personalize the thiopurine dosage to individual patient needs. By accurately identifying the patient’s TPMT isoform, clinicians can avoid toxic effects and prevent life-threatening conditions.

Trust in Viennalab’s expertise and experience in molecular diagnostics to provide you with reliable and easy-to-use results for your laboratory workflow. Take the first step towards achieving personalized thiopurine-based therapy and improving patient outcomes – add the Viennalab TPMT StripAssay® Kit to your laboratory today.

Components of the TPMT Stripassay
  1. DNA Extraction Kit: A kit containing all the reagents necessary for the extraction of genomic DNA from a blood or saliva sample, including DNA extraction buffer, proteinase K, and magnetic beads.
  2. StripAssay: A test strip containing immobilized probes specific for the detection of SNPs in the TPMT gene.
  3. Conjugate: A solution containing probes labeled with biotin that bind to the immobilized probes on the StripAssay.
  4. Enzyme Conjugate: A solution containing streptavidin conjugated to horseradish peroxidase (HRP) that binds to the biotin-labeled probes on the StripAssay.
  5. Substrate: A solution containing a chromogenic substrate for HRP that produces a color reaction upon cleavage by the enzyme.
  6. Stop Solution: A solution used to stop the enzymatic reaction and stabilize the color reaction.
  7. Control DNA: A synthetic DNA that is added to the sample as an internal control to ensure that the assay is working correctly.
  8. DNase/RNase-free Water: Water that has been treated to remove contaminating DNases and RNases, which can degrade the extracted DNA.

 

The kit also includes instructions for use, as well as a protocol for the detection of SNPs in the TPMT gene using the StripAssay format.

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