Action Oncology KitDx (Automatic)
Action Oncology KitDx is a next-generation sequencing panel for solid tumours. It covers 55 genes including hotspot regions and fusion genes, contains a 110-microsatellite panel for microsatellite instability testing, detects copy number variations including 1p/19q and 1q/16p, and analyses 20 genes for pharmacogenetics. One kit covers 24 reactions. CE marked for in vitro diagnostic use.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
- 55-gene panel with hotspot regions and fusion genes
- 110-microsatellite panel for microsatellite instability testing
- Copy number variations including 1p/19q and 1q/16p, validated by a low-density SNP array
- 20 genes analysed for pharmacogenetics
- Molecular barcoding to remove PCR duplicates from the analysis
- 50 to 200 ng of DNA from peripheral blood or tissue
- 24 reactions per kit, with 24 unique 8-nucleotide indexes
Action Oncology KitDx characterises solid tumours by next-generation sequencing, covering sequence variants, microsatellite instability, copy number variations and pharmacogenetics in one workflow.
Panel content
The panel covers 55 genes, together with hotspot regions and fusion genes.
Microsatellite instability
A 110-microsatellite panel is included for microsatellite instability testing, which is used in diagnosis and prognosis.
Copy number variations
The panel detects copy number variations, including combined alterations such as 1p/19q and 1q/16p. A low-density SNP array is used to validate these results.
Pharmacogenetics
Twenty genes are analysed for pharmacogenetic variants, which provide a basis for dose adjustment in chemotherapy.
Sensitivity
Molecular barcoding is applied during library preparation, so PCR duplicates can be removed during bioinformatic analysis.
Sample and input
The assay runs on DNA from peripheral blood or from tissue, and requires 50 to 200 ng of DNA.
Workflow
Library preparation uses Illumina adapters. The kit supplies 24 unique 8-nucleotide indexes in single-use strips, so 24 samples can be identified within one sequencing run.
Quality and regulatory status
The manufacturer, Health in Code, is certified against UNE-EN ISO 13485:2018 for quality management. This kit is CE marked for in vitro diagnostic use.
The kit contains the necessary reagents to perform 24 reactions. The reagents included in the kit are the following:
- Fragmentation Buffer: Buffer used for DNA fragmentation, prior to NGS libraries preparation.
- Fragmentation Enzyme: Enzyme used for DNA fragmentation, prior to NGS libraries preparation.
- Reagents Plate: Plate with all the reagents required to perform the DNA end repair, the Illumina adapters linkage, and amplifications done during the library preparation protocol.
- Beads and Buffers plate: Plate with magnetic particles and wash buffers, required for the DNA capture and its purification during the library preparation protocol.
- Index: Oligonucleotides with a unique 8 nucleotides identifier sequence compatible with the illumina adapters required to identify each sample during library preparation and NGS sequencing.
The kit includes 24 different indexes, prepared in single-use strips. - Hematology Probes Strips: Biotinylated synthetic oligonucleotides complementary to the target regions of the target genes.
- Elution Buffer: Buffer used to elute the DNA.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
MSDS Action Oncology KitDx
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Action Oncology KitDx (Automatic).
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
