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Knowledge hub · From the bench

Method & validation notes, written for the lab

Short, applied write-ups on the assays and instruments we distribute, sample types, internal controls, protocol detail and how each method fits the workflow you already run. No marketing, just the answers labs actually ask before they validate.

The library

Browse application notes

Filter by technique or diagnostic area, or search a target, pathogen or platform.

Technique
Area
10 notes
NGS · Workflow 7 min read

Getting extraction input right before you commit an NGS run

NGS is only as good as what goes in. How nucleic-acid yield, purity and integrity shape library quality, and the pre-analytical checks worth running before you commit a flow cell.

Workflow note · Viennalab · 16S Microbiome + ITS NGS AssayRead note
Serology · Parasitology 6 min read

Reading ELISA cut-offs in parasitology serology

From plate to call: how to set and interpret cut-offs on a parasitology IgG ELISA, the role of grey-zone results, and what a positive genuinely tells you about exposure versus active disease.

Serology note · Bordier · Leishmania infantum IgG ELISA KitRead note
Real-Time PCR · Infectious 5 min read

Multiplexing chlamydia and gonorrhoeae in one PCR run

Dual-target detection of Chlamydia trachomatis and Neisseria gonorrhoeae from a single sample, why combined STI testing outperforms ordering the two assays separately.

Application note · Presto · Presto 100 CT/NG PCR KitRead note
Real-Time PCR · QC 4 min read

Reading the internal control: what RNase P tells you

A co-amplified RNase P (RPP30) internal control reports more than a passing run, how to read it, and what it flags when a result comes back negative.

QC note · Healthincode · SARS CoV 2 RNaseP KitRead note
LAMP · Infectious 5 min read

LAMP for malaria: a 30-minute isothermal answer

How the Ampliflash Malaria LAMP kit detects Plasmodium without thermal cycling, a fast, low-instrument route to a result, and where it complements rather than replaces microscopy.

Method note · BioSynex · Ampliflash® Malaria LAMP kitRead note
Extraction · Workflow 5 min read

Integrating automated extraction ahead of PCR

How the SwiftXtractor SL fits in front of common Real-Time PCR platforms, automated nucleic-acid extraction, lower hands-on time, and cleaner input for downstream amplification.

Workflow note · Xpedite · SwiftXtractor™ SLRead note
Real-Time PCR · Mycology 5 min read

Screening for Candida auris colonisation

A single-target Real-Time PCR approach to Candida auris screening, sample types, the role of rapid detection in infection control, and how molecular screening supports outbreak surveillance.

Method note · AMD · Candida auris PCR KitRead note
PCR · Syndromic 6 min read

A multi-target meningitis panel from CSF

Bacterial and fungal targets from cerebrospinal fluid in a single PCR panel, why syndromic testing shortens the path to a result when every hour of diagnostic delay carries clinical weight.

Application note · TruPCR® · Bacterial & Fungal Meningitis PCR Panel KitRead note
Real-Time PCR · Syndromic 7 min read

When to reach for a 13-target respiratory panel

SARS-CoV-2, influenza A/B and ten further respiratory targets in a single run, why a broad syndromic panel answers more of the differential than single-target testing in respiratory season.

Application note · Igenesis · iCassette – 13 Respiratory PathogensRead note
Real-Time PCR · Infectious 6 min read

Detecting hantavirus when serology is inconclusive

Real-Time PCR detection of Hantavirus RNA supports diagnosis in the acute phase, when serology has not yet seroconverted or cross-reactivity clouds the picture.

Method note · AMD · Hantavirus PCR KitRead note
No notes match those filters

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How they’re written

What you’ll find in every note

Each note is short and applied, written by people who run the assays, structured so you can scan it in minutes.

Method & sample types

The technique, the targets and the validated sample types, serum, plasma, urine, swab or CSF, so you know what fits before you order.

Controls & interpretation

Internal controls, channel allocation and how to read a valid versus invalid result, the QC detail that keeps reporting defensible.

Workflow integration

How the method sits in the chain from sample to result, compatible platforms, hands-on time and where it pairs with extraction and confirmation.

Applications consultation

Book a 15-minute applications call

When a note doesn’t quite answer your question, talk it through. Bring the targets, sample types or platform you’re working with and one of our specialists will help you choose, verify or integrate the right assay.

  • Method selection for your sample types and platform
  • Verification, controls and acceptance criteria
  • Fitting extraction, amplification and confirmation together
How it works
  • 1
    Tell us the contextTargets, sample types and the platform you run, a short message is enough to prepare.
  • 2
    We match a specialistMolecular, immuno or extraction, you talk to someone who knows the chemistry.
  • 3
    15 focused minutesA clear, practical answer, and the documentation or a quote to follow if you need it.
Let’s talk diagnostics

A question the notes don’t answer?

Tell us the assays, platforms or targets you’re working with and our specialists will respond with practical guidance, and a quote and the right documentation where you need them. No public pricing, just a clear answer.