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The Brucella spp. PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the qualitative detection of Brucella DNA in human clinical samples. Based on advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly specific and reliable identification of Brucella species, the causative agents of brucellosis.
Targeting conserved regions within the Brucella genome, the kit enables accurate detection even in low-copy samples. An integrated internal control monitors DNA quality and detects potential PCR inhibition, ensuring confidence in diagnostic results.
With robust assay performance and compatibility with widely used real-time PCR platforms, this kit is ideal for clinical diagnostics and research laboratories focused on zoonotic infectious diseases.
The Brucella spp. PCR Detection Kit is an in-vitro real-time PCR assay developed for the qualitative identification of Brucella DNA in human samples. Utilizing hydrolysis probe-based detection, the assay delivers high sensitivity and specificity for this clinically significant zoonotic pathogen.
The kit includes a ready-to-use master mix containing primers and probes for both Brucella detection and an internal control gene, enabling simultaneous validation of sample quality and amplification efficiency.
Brucella spp. are small, Gram-negative, facultative intracellular bacteria responsible for brucellosis, a zoonotic disease transmitted primarily through consumption of unpasteurized dairy products or direct contact with infected animals.
Human infection typically presents with non-specific symptoms such as fever, fatigue, joint pain, and hepatosplenomegaly. Chronic or relapsing infections may lead to complications affecting the musculoskeletal, nervous, or cardiovascular systems.
Due to the intracellular nature of Brucella and limitations of culture-based diagnostics, rapid and accurate molecular detection is essential for effective diagnosis, treatment, and public health control.
The Brucella spp. PCR Detection Kit is designed for reliable performance in routine diagnostic workflows. The assay uses dual-channel fluorescence detection: the FAM channel detects Brucella DNA, while the HEX channel serves as an internal control to verify DNA quality and detect PCR inhibition.
The assay incorporates “hot start” PCR technology to minimize non-specific amplification and improve sensitivity. Uracil-DNA glycosylase (UDG) is included to prevent carry-over contamination, ensuring consistent and reproducible results.
With high analytical sensitivity and specificity under validated conditions, the kit enables accurate detection even in low-level infections. It is suitable for a range of specimen types, including whole blood, serum, and other validated clinical samples.
Instructions for Use