Brucella spp. PCR Kit
The Brucella spp. PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the qualitative detection of Brucella DNA in human clinical samples. Based on advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly specific and reliable identification of Brucella species, the causative agents of brucellosis. Targeting conserved regions within the Brucella genome, the kit enables accurate detection even in low-copy samples. An integrated internal control monitors DNA quality and detects potential PCR inhibition, ensuring confidence in diagnostic results. With robust assay performance and compatibility with widely used real-time PCR platforms, this kit is ideal for clinical diagnostics and research laboratories focused on zoonotic infectious diseases.
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This kit in detail
The Brucella spp. PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the qualitative detection of Brucella DNA in human clinical samples. Based on advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly specific and reliable identification of Brucella species, the causative agents of brucellosis.
Targeting conserved regions within the Brucella genome, the kit enables accurate detection even in low-copy samples. An integrated internal control monitors DNA quality and detects potential PCR inhibition, ensuring confidence in diagnostic results.
With robust assay performance and compatibility with widely used real-time PCR platforms, this kit is ideal for clinical diagnostics and research laboratories focused on zoonotic infectious diseases.
- High sensitivity detection of Brucella DNA
- Dual-channel FAM/HEX with internal control
- High specificity for Brucella spp.
- Ready-to-use, contamination-controlled workflow
Qualitative PCR Detection of Brucella spp.
The Brucella spp. PCR Detection Kit is an in-vitro real-time PCR assay developed for the qualitative identification of Brucella DNA in human samples. Utilizing hydrolysis probe-based detection, the assay delivers high sensitivity and specificity for this clinically significant zoonotic pathogen.
The kit includes a ready-to-use master mix containing primers and probes for both Brucella detection and an internal control gene, enabling simultaneous validation of sample quality and amplification efficiency.
Molecular Background
Brucella spp. are small, Gram-negative, facultative intracellular bacteria responsible for brucellosis, a zoonotic disease transmitted primarily through consumption of unpasteurized dairy products or direct contact with infected animals.
Human infection typically presents with non-specific symptoms such as fever, fatigue, joint pain, and hepatosplenomegaly. Chronic or relapsing infections may lead to complications affecting the musculoskeletal, nervous, or cardiovascular systems.
Due to the intracellular nature of Brucella and limitations of culture-based diagnostics, rapid and accurate molecular detection is essential for effective diagnosis, treatment, and public health control.
Assay Performance & Utility
The Brucella spp. PCR Detection Kit is designed for reliable performance in routine diagnostic workflows. The assay uses dual-channel fluorescence detection: the FAM channel detects Brucella DNA, while the HEX channel serves as an internal control to verify DNA quality and detect PCR inhibition.
The assay incorporates “hot start” PCR technology to minimize non-specific amplification and improve sensitivity. Uracil-DNA glycosylase (UDG) is included to prevent carry-over contamination, ensuring consistent and reproducible results.
With high analytical sensitivity and specificity under validated conditions, the kit enables accurate detection even in low-level infections. It is suitable for a range of specimen types, including whole blood, serum, and other validated clinical samples.
Technical Specifications
- Assay Type: Qualitative Real-Time PCR (qPCR)
- Target: Brucella spp. DNA
- Detection Channels: FAM (Brucella spp.), HEX (Internal Control)
- Sensitivity: High analytical sensitivity (validated in-house)
- Sample Types: Whole blood, serum, and other validated clinical samples
- Input Material: Extracted DNA
- Compatible Instruments: ABI 7500, QuantStudio 3/5/7, Bio-Rad CFX, Rotor-Gene Q, LightCycler 480, and equivalent systems
- Brucella spp. qPCR Master Mix
- Brucella spp. Positive Control
- Nuclease-free Water
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Brucella spp. PCR Kit.
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