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The TRUPCR® Carbapenem Resistance Detection Kit is a real-time PCR assay designed for the qualitative detection and differentiation of the most clinically important carbapenemase genes: blaKPC, blaNDM, blaVIM, blaOXA-48-like, and blaIMP. These resistance determinants are among the primary drivers of carbapenem non-susceptibility in Gram-negative pathogens, making their rapid identification essential for effective infection control and antimicrobial stewardship.
Using multiplex TaqMan® probe chemistry, the kit delivers high analytical specificity and sensitivity, enabling reliable detection even at low bacterial loads. Each reaction contains an integrated internal control to verify amplification performance and identify potential PCR inhibition, ensuring confidence in negative results.
The kit includes all necessary reagents, as well as positive and negative controls, and is validated across widely used real-time PCR instruments. With a limit of detection as low as 10²–10³ CFU/ml depending on the target gene, the TRUPCR® Carbapenem Resistance Detection Kit offers a rapid, streamlined workflow for laboratories seeking accurate identification of key carbapenem resistance mechanisms.
The TRUPCR® Carbapenem Resistance Detection Kit is designed for the qualitative detection and differentiation of the five most relevant carbapenemase gene families: blaKPC, blaNDM, blaVIM, blaOXA-48-like, and blaIMP. These gene families are associated with carbapenem non-susceptibility in Gram-negative bacteria. The assay uses real-time PCR technology to identify the presence of these targets in extracted nucleic acids. The kit provides a standardized workflow suitable for routine screening and molecular characterization of carbapenemase-producing organisms.
Carbapenemase production is one of the primary mechanisms responsible for reduced susceptibility to carbapenem antibiotics. Each of the targeted genes encodes a specific enzyme type with the ability to hydrolyze carbapenems:
Detection of these gene families supports the molecular confirmation of carbapenem resistance mechanisms. The assay identifies the genetic determinants but does not assess phenotypic susceptibility. Results should be evaluated alongside microbiological, clinical and epidemiological information.
The kit employs multiplex TaqMan® probe chemistry, enabling simultaneous detection of multiple carbapenemase targets within the same reaction setup. Each reaction incorporates an internal control to monitor for PCR inhibition and verify that a valid amplification process has occurred. The assay reagents are supplied ready-to-use, including optimized mastermix, primer–probe sets, and positive and negative controls. This design supports consistency across runs and minimizes hands-on preparation time.
During amplification, primers anneal to their respective gene targets and TaqMan® probes hybridize to complementary sequences. Probe cleavage during polymerase extension generates fluorescence proportional to the amount of target nucleic acid present. Multiplexing enables parallel detection of all carbapenemase genes included in the kit.
The workflow consists of nucleic acid extraction, reaction setup with provided reagents, real-time PCR amplification, and analysis of fluorescence signals. The integrated internal control confirms that negative results are interpretable by detecting issues such as inhibition or degraded nucleic acid.
The TRUPCR® Carbapenem Resistance Detection Kit is intended for in vitro diagnostic use in the detection of carbapenemase gene families in bacterial isolates or clinical specimens. Applications include:
The assay detects five major carbapenemase gene families; however, a negative result does not exclude the presence of other resistance mechanisms or genes not included in the panel. All results should be considered with additional laboratory data and relevant clinical context.
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