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The HLA-DQ Coeliac™ Detection Kit provides a reliable solution for the detection of HLA-DQ genotypes associated with genetic susceptibility to coeliac disease using real-time PCR. The assay enables identification of key alleles, including HLA-DQA105, HLA-DQA103, and HLA-DQB1*02/03:02, within a multiplex workflow.
An endogenous β-globin control is included to verify DNA quality and assay performance, ensuring consistent and reproducible results. The kit supports accurate assessment of genetic predisposition and enables determination of HLA-DQB1*02 zygosity.
Note that this kit is for Research Use Only (RUO).
Coeliac disease is a chronic autoimmune disorder triggered by the ingestion of gluten in genetically predisposed individuals. Gluten proteins, found in wheat, rye, and barley, induce an immune response that leads to inflammation of the small intestine and damage to the intestinal villi. This results in impaired nutrient absorption and a range of gastrointestinal and systemic symptoms.
A strong genetic component underlies coeliac disease, with the majority of patients carrying specific alleles within the human leukocyte antigen (HLA) region on chromosome 6. These genes encode proteins involved in antigen presentation and immune recognition. In coeliac disease, the most relevant genetic factors are the HLA-DQ2 and HLA-DQ8 heterodimers, which present gluten-derived peptides to CD4+ T cells and initiate the immune response.
The HLA-DQ2 heterodimer is formed by the combination of HLA-DQA105 and HLA-DQB102 alleles, while HLA-DQ8 is associated with HLA-DQA103 and HLA-DQB103:02. Approximately 90% of individuals with coeliac disease carry the HLA-DQ2 genotype, while most of the remaining patients carry HLA-DQ8. The presence of these alleles is therefore a key genetic marker for disease susceptibility.
The HLA-DQ Coeliac™ Detection Kit is designed for the qualitative detection of HLA alleles associated with coeliac disease using real-time PCR. The assay enables simultaneous detection of HLA-DQA105, HLA-DQA103, and HLA-DQB103:02 alleles, as well as discrimination of HLA-DQB102 from other HLA-DQB1 variants.
The workflow consists of multiplex PCR reactions that allow efficient amplification and detection of target alleles within a limited number of steps. This design reduces hands-on time and minimizes variability, supporting reproducible results across different laboratory settings.
In addition to allele detection, the assay enables determination of HLA-DQB1*02 zygosity, providing information on the number of risk alleles present. This is relevant for research into gene dosage effects and their association with disease susceptibility.
An endogenous β-globin gene is included as an internal control to verify DNA integrity and confirm correct assay performance. This control ensures that negative results are not due to insufficient DNA input or PCR inhibition, supporting reliable interpretation.
The inclusion of this control contributes to consistent assay performance across different sample types and experimental conditions.
The assay is designed for integration into standard molecular biology workflows and is compatible with commonly used real-time PCR platforms. It requires a low input of genomic DNA and can be applied to DNA extracted from peripheral blood or other validated sample types.
The multiplex format enables simultaneous analysis of multiple alleles in a single run, improving efficiency while maintaining analytical accuracy.
Detection of HLA-DQ genotypes is widely used in research on coeliac disease susceptibility and immune system function. Identification of HLA-DQ2 and HLA-DQ8 alleles supports studies on genetic predisposition, disease risk, and population genetics.
The ability to determine HLA-DQB1*02 zygosity enables investigation of gene dosage effects and their relationship with disease severity and progression. In addition, HLA typing contributes to research on immune-mediated diseases and antigen presentation mechanisms.
The HLA-DQ Coeliac™ Detection Kit provides a reliable and reproducible solution for the detection of HLA alleles associated with coeliac disease. Its real-time PCR-based multiplex design, internal control, and zygosity analysis enable accurate assessment of genetic susceptibility.
Designed for integration into molecular workflows, the kit supports research into autoimmune disease mechanisms, genetic risk factors, and immune system function.
This kit contains enough reagents to perform 48 real-time PCR reactions for each of the
two master mixes analyzed by this assay. The reagents included in this kit are the following: