Coxiella burnetii PCR Kit
The Coxiella burnetii PCR Detection Kit is a high-performance real-time PCR assay designed for the qualitative detection of Coxiella burnetii DNA. Based on advanced hydrolysis probe (TaqMan®) technology, the assay enables highly sensitive and specific identification of this zoonotic pathogen in veterinary and research samples. Targeting conserved regions of the C. burnetii genome, the kit ensures reliable detection across infected specimens. An integrated internal control system verifies DNA quality and identifies potential PCR inhibition, ensuring accurate and reproducible results. With optimized assay chemistry and compatibility with major real-time PCR platforms, this kit provides an efficient solution for pathogen detection and disease monitoring.
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This kit in detail
The Coxiella burnetii PCR Detection Kit is a high-performance real-time PCR assay designed for the qualitative detection of Coxiella burnetii DNA. Based on advanced hydrolysis probe (TaqMan®) technology, the assay enables highly sensitive and specific identification of this zoonotic pathogen in veterinary and research samples.
Targeting conserved regions of the C. burnetii genome, the kit ensures reliable detection across infected specimens. An integrated internal control system verifies DNA quality and identifies potential PCR inhibition, ensuring accurate and reproducible results.
With optimized assay chemistry and compatibility with major real-time PCR platforms, this kit provides an efficient solution for pathogen detection and disease monitoring.
- High sensitivity detection down to 10 copies per reaction
- High specificity for accurate C. burnetii DNA identification
- Dual-channel detection with internal control (FAM/HEX)
- Ready-to-use workflow for efficient laboratory processing
Qualitative PCR Detection of Coxiella burnetii DNA
The Coxiella burnetii PCR Detection Kit is an in-vitro real-time PCR assay developed for the qualitative identification of C. burnetii DNA. The assay utilizes hydrolysis probe technology to amplify and detect specific bacterial targets with high sensitivity and specificity.
The kit includes a ready-to-use master mix with primers and probes for target detection and internal control, enabling simultaneous validation of sample quality and amplification efficiency.
Molecular Background
Coxiella burnetii is a Gram-negative intracellular bacterium and the causative agent of Q fever, a globally distributed zoonotic disease. The pathogen primarily infects livestock such as goats, sheep, and cattle, and is shed in birth products, milk, urine, and feces.
Transmission to humans and other animals typically occurs through inhalation of contaminated aerosols or dust particles. In animals, infection can lead to reproductive disorders such as abortion, infertility, stillbirth, and mastitis, resulting in significant economic impact.
Assay Performance & Utility
The assay is designed for reliable detection of C. burnetii DNA in a variety of sample types. Real-time PCR ensures rapid and accurate identification, even at low bacterial loads.
Hot start PCR technology minimizes non-specific amplification, while uracil-DNA glycosylase (UDG) reduces the risk of carry-over contamination. The dual-channel detection system enables clear result interpretation, with target detection and internal control validation.
Technical Specifications
- Assay Type: Qualitative Real-Time PCR (qPCR)
- Target: Coxiella burnetii DNA
- Detection Channels: FAM (target), HEX (internal control)
- Sensitivity: ≥10 copies per reaction
- Sample Types: Veterinary and environmental samples (validated in-house)
- Input Material: Extracted DNA
- Technology: One-step qPCR with hydrolysis probe detection
- Compatible Instruments: ABI 7500, QuantStudio series, Bio-Rad CFX, Rotor-Gene Q, LightCycler 480, and equivalent systems
- qPCR Master Mix
- Positive control
- Nuclease-free water
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Documentation available on request, contact us and we will send it over.
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Coxiella burnetii PCR Kit.
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Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
