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The Coxiella burnetii PCR Detection Kit is a high-performance real-time PCR assay designed for the qualitative detection of Coxiella burnetii DNA. Based on advanced hydrolysis probe (TaqMan®) technology, the assay enables highly sensitive and specific identification of this zoonotic pathogen in veterinary and research samples.
Targeting conserved regions of the C. burnetii genome, the kit ensures reliable detection across infected specimens. An integrated internal control system verifies DNA quality and identifies potential PCR inhibition, ensuring accurate and reproducible results.
With optimized assay chemistry and compatibility with major real-time PCR platforms, this kit provides an efficient solution for pathogen detection and disease monitoring.
The Coxiella burnetii PCR Detection Kit is an in-vitro real-time PCR assay developed for the qualitative identification of C. burnetii DNA. The assay utilizes hydrolysis probe technology to amplify and detect specific bacterial targets with high sensitivity and specificity.
The kit includes a ready-to-use master mix with primers and probes for target detection and internal control, enabling simultaneous validation of sample quality and amplification efficiency.
Coxiella burnetii is a Gram-negative intracellular bacterium and the causative agent of Q fever, a globally distributed zoonotic disease. The pathogen primarily infects livestock such as goats, sheep, and cattle, and is shed in birth products, milk, urine, and feces.
Transmission to humans and other animals typically occurs through inhalation of contaminated aerosols or dust particles. In animals, infection can lead to reproductive disorders such as abortion, infertility, stillbirth, and mastitis, resulting in significant economic impact.
The assay is designed for reliable detection of C. burnetii DNA in a variety of sample types. Real-time PCR ensures rapid and accurate identification, even at low bacterial loads.
Hot start PCR technology minimizes non-specific amplification, while uracil-DNA glycosylase (UDG) reduces the risk of carry-over contamination. The dual-channel detection system enables clear result interpretation, with target detection and internal control validation.
Instructions for Use