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Cytomegalovirus PCR Detection Kit
The TRUPCR® CMV QT PCR Kit is a real-time PCR assay designed for the qualitative detection and quantification of Cytomegalovirus (CMV) DNA in human plasma samples. This in vitro diagnostic solution amplifies a conserved region of the CMV genome using hot-start technology for maximum specificity. The assay includes an internal control (IC) to monitor PCR performance and reduce the risk of false negatives. With superior sensitivity compared to serological and culture-based methods, this kit offers rapid, reliable, and reproducible detection of CMV-especially essential for managing immunocompromised patients and monitoring transplant recipients. It is fully compatible with major PCR platforms and is standardized against the 1st WHO International Standard for CMV nucleic acid amplification (NIBSC code 09/162).
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This kit in detail
The TRUPCR® CMV QT PCR Kit is a real-time PCR assay designed for the qualitative detection and quantification of Cytomegalovirus (CMV) DNA in human plasma samples. This in vitro diagnostic solution amplifies a conserved region of the CMV genome using hot-start technology for maximum specificity. The assay includes an internal control (IC) to monitor PCR performance and reduce the risk of false negatives. With superior sensitivity compared to serological and culture-based methods, this kit offers rapid, reliable, and reproducible detection of CMV-especially essential for managing immunocompromised patients and monitoring transplant recipients. It is fully compatible with major PCR platforms and is standardized against the 1st WHO International Standard for CMV nucleic acid amplification (NIBSC code 09/162).
- Accurate Cytomegalovirus detection and quantification
- WHO-standardized and validated
- Built-in internal control ensures test reliability
- Compatible with leading real-time PCR systems
Cytomegalovirus PCR Kit
What is in the Cytomegalovirus PCR Kit?
The TRUPCR® Cytomegalovirus (CMV) QT PCR Kit includes reagents and primers targeting highly conserved regions of the CMV genome. It features two parallel reactions per well:
- CMV DNA detection (FAM channel)
- Internal Control (IC) (Texas Red channel)
This configuration allows for accurate detection of viral DNA while excluding false negatives caused by PCR inhibition or extraction errors. The kit also includes components for reaction setup and quantification, ensuring reproducibility across clinical labs.
Role of Cytomegalovirus in Disease
Cytomegalovirus (CMV), also known as Human Herpesvirus 5 (HHV-5), is a member of the Herpesviridae family. With a genome size of 235 kb, CMV is the largest and one of the most complex human herpesviruses. Its prevalence is widespread, affecting 40–100% of immunocompetent adults worldwide.
While many CMV infections remain asymptomatic, it poses significant risks in:
- Immunocompromised patients (e.g., transplant recipients, HIV patients)
- Congenital infections with long-term developmental impacts
- Reactivated infections in critically ill patients
The virus is commonly found in bodily fluids including urine, saliva, blood, semen, and breast milk. Routine screening is essential in settings where rapid intervention is crucial to prevent complications like CMV-positive organ rejection or life-threatening viremia.
About the TRUPCR® CMV QT PCR Kit
The TRUPCR® CMV QT Kit is engineered for clinical diagnostic labs requiring high-throughput and reliable CMV DNA quantification. Its robust design includes hot-start Taq polymerase to eliminate non-specific amplifications and minimize background noise. Calibrated against the 1st WHO International Standard, it ensures global consistency in CMV viral load reporting.
Built-in internal control DNA helps detect PCR inhibition and confirms sample integrity. The kit’s compatibility with mainstream qPCR instruments (e.g., ABI 7500, CFX96, QuantStudio®) allows seamless integration into routine workflows.
Principle and Workflow
The test uses a TaqMan-based real-time PCR approach with hot-start chemistry. Workflow includes:
- Extracting nucleic acid from plasma samples.
- Preparing reaction mix with master mix, primers, and template.
- Running the reaction on a compatible qPCR machine.
- Monitoring fluorescence from FAM (CMV) and Texas Red (IC) channels.
The process is streamlined and efficient, with total runtime under 2 hours. Data output includes quantitation of CMV DNA and IC performance, delivering dependable results every time.
Clinical Application
The TRUPCR® CMV QT PCR Kit is a critical tool for:
- Routine CMV monitoring in solid organ and stem cell transplant programs
- Diagnosing primary or reactivated CMV infections
- Assessing CMV-related complications in HIV and oncology patients
- Supporting clinical trials focused on antiviral therapies
The assay helps differentiate between latent and active infections, enabling evidence-based antiviral treatment. By offering quantification, it also assists in tracking disease progression and therapeutic response.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Product Catalogus
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