EGFR Real-time PCR Kit, tap a thumbnail to see more views.
EGFR Real-time PCR Kit
The TruPCR EGFR Mutation Kit detects 32 mutations across exons 18, 19, 20 and 21 of the EGFR gene by allele-specific ARMS real-time PCR, with a stated sensitivity of 1 per cent mutant DNA. The panel includes the exon 19 deletions, p.L858R and the resistance mutation p.T790M. In non-small cell lung cancer these results determine the choice of tyrosine kinase inhibitor. DNA from fresh, frozen or formalin-fixed paraffin-embedded tissue is used.
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This kit in detail
- 32 EGFR mutations across exons 18, 19, 20 and 21
- Includes the exon 19 deletions, p.L858R and p.T790M
- ARMS PCR with selective amplification of the mutant allele
- Sensitivity 1 per cent mutant DNA
- Internal extraction control against false negatives
- 24 or 48 reactions per kit, REF 3B1311 and 3B1312
The TruPCR EGFR Mutation Kit determines the EGFR mutation status of a lung tumour, the result that decides whether a tyrosine kinase inhibitor is an option and which generation of inhibitor applies.
Clinical background
EGFR is a receptor tyrosine kinase that regulates cell growth and survival. In non-small cell lung cancer, activating mutations keep the receptor switched on: the deletions in exon 19 and p.L858R in exon 21 account for most of them and predict a response to tyrosine kinase inhibitors. p.T790M in exon 20 is the resistance mutation that appears under treatment with a first or second generation inhibitor, and it dictates the switch to a third generation drug.
Method and sensitivity
The assay uses ARMS PCR, in which the primer amplifies only the mutant allele, so a small mutant fraction can be detected against a wild-type background. The stated sensitivity is 1 per cent. An internal extraction control is amplified alongside, so a negative result can be distinguished from a failed extraction. DNA from fresh, frozen or formalin-fixed paraffin-embedded tumour tissue is used.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Product Catalogus
Frequently paired with this kit
Other assays and instruments lab teams add alongside the EGFR Real-time PCR Kit.
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