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The TRUPCR® ESKAPE ID & AMR Panel Kit is a real-time PCR–based assay designed for the rapid identification of highly virulent ESKAPE pathogens and the simultaneous detection of key antimicrobial resistance genes. Targeting Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species, the panel supports fast and accurate profiling of organisms responsible for the majority of nosocomial infections. Its multiplex architecture enables differentiation of AMR markers associated with extended-spectrum β-lactams, carbapenems, colistin, vancomycin, and methicillin within a single workflow.
With 18 clinically relevant targets consolidated into six reactions, the assay provides actionable results to support infection control strategies and early therapeutic decision-making. The kit features a limit of detection of 10 copies per reaction and incorporates an internal control to verify amplification efficiency and sample integrity. Ready-to-use reagents and compatibility with widely used PCR instruments streamline setup and reduce handling time, making the TRUPCR® ESKAPE ID & AMR Panel Kit a practical tool for high-throughput diagnostic environments.
For Research Use Only (RUO)
The TRUPCR® ESKAPE ID & AMR Panel Kit offers a comprehensive molecular solution for identifying the most prevalent nosocomial pathogens and their associated antimicrobial resistance mechanisms. By integrating organism identification with AMR gene detection in one workflow, the assay supports rapid decision-making in settings where timely infection control and targeted therapy are critical. The panel is specifically designed to address the challenges posed by ESKAPE pathogens, which are known for their virulence, high transmission potential, and increasing ability to evade standard antimicrobial treatments.
ESKAPE organisms (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) represent a major global health concern due to their role in hospital-acquired infections and their ability to develop multidrug resistance. These pathogens are capable of escaping the effects of commonly used antibiotics through mechanisms such as β-lactamase production, carbapenemase activity, alteration of drug targets, and resistance gene acquisition via mobile genetic elements. Rapid characterization of both organism identity and resistance profile is therefore essential to limit spread, improve clinical outcomes, and support antimicrobial stewardship initiatives.
The TRUPCR® ESKAPE ID & AMR Panel Kit targets a comprehensive set of AMR markers associated with extended-spectrum β-lactams, carbapenems, colistin, vancomycin, and methicillin resistance, enabling a broad assessment of clinically actionable resistance determinants within the same assay run.
The TRUPCR® ESKAPE ID & AMR Panel Kit uses multiplex real-time PCR for the qualitative detection of ESKAPE pathogens and differentiation of their associated AMR genes. The panel consolidates 18 targets into six reactions, providing a streamlined workflow without compromising analytical depth. Each reaction includes a built-in internal control to monitor PCR inhibition, verify sample processing, and ensure valid amplification performance.
The ready-to-use reagent system simplifies laboratory handling, reducing setup time while maintaining consistency between runs. The assay is intended for use with nucleic acid extracted from clinical specimens or cultured isolates. As with all molecular diagnostic assays, results should be interpreted alongside clinical findings, bacterial culture data, and epidemiological context.
The assay relies on fluorescent hydrolysis probe chemistry for target detection. During amplification, pathogen- or resistance-specific probes hybridize to their complementary sequences. Probe cleavage by DNA polymerase separates the fluorophore from the quencher, generating an accumulating fluorescent signal that correlates with the presence of the target sequence. Multiplexing allows simultaneous detection of multiple signatures within the same reaction.
The workflow consists of DNA extraction, reaction setup with pre-formulated primer–probe mixes, real-time PCR amplification, and automated interpretation of amplification curves. The inclusion of an internal control in every tube ensures that negative results are meaningful and that inhibitors, sample degradation, or procedural errors are identifiable.
The TRUPCR® ESKAPE ID & AMR Panel Kit is designed to support early detection of high-priority pathogens and rapid assessment of clinically significant AMR markers. By providing both organism ID and resistance information within the same analytical run, laboratories can deliver actionable insights for infection control teams, antimicrobial stewardship programs, and frontline clinicians. This is particularly valuable in high-risk patient populations, including those in intensive care units, oncology wards, and long-term care environments.
For Research Use Only (RUO)
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