Factor XII PCR Kit
Factor XII is a molecular biology assay designed for the analysis of genetic variation in the F12 gene. The assay utilizes real-time PCR technology to enable sensitive and specific detection of target sequences within a streamlined workflow. The kit is optimized for efficient processing and requires a minimal amount of genomic DNA input, supporting consistent performance across standard laboratory conditions. It is compatible with commonly used real-time PCR platforms, allowing straightforward integration into existing molecular biology workflows. Note that this kit is for Research Use Only (RUO)
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
Factor XII is a molecular biology assay designed for the analysis of genetic variation in the F12 gene. The assay utilizes real-time PCR technology to enable sensitive and specific detection of target sequences within a streamlined workflow.
The kit is optimized for efficient processing and requires a minimal amount of genomic DNA input, supporting consistent performance across standard laboratory conditions. It is compatible with commonly used real-time PCR platforms, allowing straightforward integration into existing molecular biology workflows.
Note that this kit is for Research Use Only (RUO)
- Dual allele detection in a single PCR reaction
- Real-time PCR with fluorescent probe technology
- Includes heterozygous positive control
- Compatible with standard real-time PCR platforms
Factor XII: Advanced Genetic Analysis
The Factor XII assay is designed for the analysis of genetic variation in the F12 gene using real-time PCR technology. It enables the detection of both the c.46C>T (NM_000505.3: c.-4T>C) variant and the corresponding wild-type allele within a single PCR reaction.
Background
The F12 gene encodes coagulation factor XII, a protein involved in the initiation of the intrinsic coagulation pathway. Genetic variation in this gene is commonly studied in relation to coagulation mechanisms and laboratory parameters.
Workflow and Application
The assay is optimized for efficient processing and requires a low input of genomic DNA. Its single-reaction format supports streamlined workflows and consistent performance in standard laboratory settings. The inclusion of a positive control supports assay performance and result interpretation.
Conclusion
Factor XII provides a reliable solution for the analysis of genetic variants in the F12 gene. Its compatibility with standard real-time PCR platforms supports consistent and reproducible results in molecular biology workflows.
The kit contents the necessary reagents to perform 48 reactions. The reagents included in the kit are the following:
- Factor XII Master Mix: contains oligonucleotides, fluorescent hydrolysis probes (FAM and VIC) and water for the amplification and detection of the normal and/or mutant alleles under analysis.
- General Master Mix: PCR Master Mix with nucleotides, MgCl2, enzyme and buffer to carry out real-time PCR.
- Positive Control: positive control for the simultaneous amplification of the normal allele and the mutant allele under analysis (simulating a heterozygous sample).
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
MSDS Factor XII PCR Kit
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Factor XII PCR Kit.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
