FLT3 Gene Kit

Background on FLT3 and Its Role in AML

The FLT3 gene encodes a receptor tyrosine kinase that plays a key role in hematopoietic stem cell proliferation and differentiation. Mutations in FLT3, particularly internal tandem duplications (ITD) and point mutations in the tyrosine kinase domain (TKD), are among the most common genetic alterations in Acute Myeloid Leukemia (AML). These mutations are associated with increased proliferation of leukemic blasts, disease progression, and poor prognosis.

Detection and characterization of FLT3 mutations is essential for research into AML pathogenesis, genotype-phenotype correlations, and the development of targeted therapeutic approaches. Accurate identification of FLT3 ITD length, allelic ratio, and TKD mutations is critical for understanding disease biology and for preclinical studies of FLT3-targeted inhibitors.

Kit Description

The FLT3™ Gene Mutation Detection Kit provides a research-grade solution for the rapid and reliable detection of FLT3 gene mutations, including ITDs and TKD variants. The assay combines PCR amplification with capillary electrophoresis to precisely identify mutation type, size, and allelic ratio. The kit is designed for molecular biology research use, enabling accurate characterization of FLT3 in AML samples and experimental models.

The kit’s workflow consolidates amplification and fragment analysis into a single streamlined protocol, reducing hands-on time, minimizing potential sources of variability, and supporting reproducible results across multiple runs and laboratories.

Integrated Positive Control

An integrated positive control is included to verify assay performance and to support accurate result interpretation. The control contains both the normal FLT3 sequence and a 39-bp ITD reference sequence, ensuring reliable detection across a range of mutation types and sample qualities. This provides confidence in data reproducibility and reduces the risk of false negatives, particularly in samples with low mutant allele burden.

Reagents and Assay Performance

All components of the FLT3™ Gene Mutation Detection Kit are manufactured to strict quality standards. Primers, PCR reagents, and capillary electrophoresis materials are optimized for high sensitivity and specificity, allowing detection of mutations at allele frequencies as low as 5%. The assay covers a broad amplification range, typically 100–500 bp, to capture various ITD sizes and TKD variants.

The kit has been validated using reference materials, synthetic constructs, and biobank specimens. This validation demonstrates robust, reproducible performance and ensures that laboratories can rely on accurate detection of FLT3 mutations in research settings.

Research and Biological Significance

FLT3 mutations, especially ITDs, drive constitutive activation of the receptor’s kinase activity, leading to enhanced JAK-STAT and PI3K signaling. These mutations are critical biomarkers for AML prognosis, stratification, and potential response to targeted therapy. Reliable detection allows researchers to quantify allelic burden, monitor mutation dynamics, and study correlations between mutation characteristics and disease phenotype.

The kit enables comprehensive studies of FLT3 mutation biology, including experimental models of AML, investigations into mutation-driven signaling pathways, and preclinical evaluation of FLT3 inhibitors. Accurate characterization of FLT3 ITD length and allelic ratio is particularly important for translational research and understanding treatment resistance mechanisms.

Summary

The FLT3™ Gene Mutation Detection Kit provides a complete, research-grade solution for detecting and characterizing FLT3 mutations in AML. Its combined PCR and capillary electrophoresis workflow, integrated positive control, and validated reagents ensure accurate, reproducible results with minimal procedural complexity. Optimized for integration into molecular biology workflows, the kit supports high-throughput research while maintaining analytical precision.

By enabling reliable detection of FLT3 ITD and TKD mutations, including allelic ratio quantification, the kit empowers laboratories to advance AML research, investigate prognostic biomarkers, and study therapeutic targets. The FLT3™ Gene Mutation Detection Kit delivers the sensitivity, reliability, and quality required for comprehensive molecular studies, supporting scientific insight into AML pathophysiology and future treatment strategies.

The kit contents the necessary reagents to perform 33 reactions. The reagents included in the kit are the following:

• FLT3 Master Mix: Buffer, MgCl2, dNTPs, DMSO, specific oligonucleotides and water reagents
  for the PCR reactions.
• Taq: Conventional PCR enzyme containing 50% glycerol and buffers.
• Positive Control: Positive control with the wild type of sequence of FLT3 and an internal
  tandem duplication (ITD) of 39pb in the same gene.