X
- No products in the list
The Gardnerella vaginalis (G. vaginalis) PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the qualitative detection of G. vaginalis DNA in human clinical samples. Based on advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly specific and reliable identification of this key bacterium associated with bacterial vaginosis (BV).
Targeting conserved regions of the G. vaginalis genome, including virulence-associated genes, the kit enables accurate detection even in low-copy samples. An integrated internal control monitors DNA quality and detects potential PCR inhibition, ensuring confidence in diagnostic results.
With optimized assay chemistry and compatibility with widely used real-time PCR platforms, this kit is ideal for clinical diagnostics and research focused on women’s health and microbiome-related conditions.
The G. vaginalis PCR Detection Kit is an in-vitro diagnostic real-time PCR assay developed for the qualitative identification of G. vaginalis DNA in human samples. Utilizing hydrolysis probe-based detection, the assay delivers high sensitivity and specificity for this clinically relevant bacterium.
The kit includes a ready-to-use master mix containing primers and probes for both G. vaginalis detection and an internal control gene, enabling simultaneous validation of sample quality and amplification efficiency.
Gardnerella vaginalis is a facultatively anaerobic, Gram-variable bacterium commonly associated with bacterial vaginosis, a condition characterized by disruption of the normal vaginal microbiota. Overgrowth of G. vaginalis leads to a reduction in protective Lactobacillus species and an increase in vaginal pH.
This imbalance may result in symptoms such as abnormal discharge and odor, although infections can also be asymptomatic. G. vaginalis contributes to biofilm formation on the vaginal epithelium, which increases resistance to treatment and contributes to recurrence.
Accurate molecular detection is important for diagnosis, treatment monitoring, and understanding microbiome-related conditions.
The G. vaginalis PCR Detection Kit is designed for reliable performance in routine diagnostic workflows. The assay uses dual-channel fluorescence detection: the FAM channel detects G. vaginalis DNA, while the HEX channel serves as an internal control to verify DNA quality and detect PCR inhibition.
The assay incorporates “hot start” PCR technology to minimize non-specific amplification and enhance sensitivity. Uracil-DNA glycosylase (UDG) is included to prevent carry-over contamination, ensuring consistent and reproducible results.
With high analytical sensitivity and near 100% specificity under validated conditions, the kit enables accurate detection across a range of sample types, particularly vaginal and cervical swabs.
Instructions for Use