Gardnerella vaginalis PCR Kit
The Gardnerella vaginalis (G. vaginalis) PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the qualitative detection of G. vaginalis DNA in human clinical samples. Based on advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly specific and reliable identification of this key bacterium associated with bacterial vaginosis (BV). Targeting conserved regions of the G. vaginalis genome, including virulence-associated genes, the kit enables accurate detection even in low-copy samples. An integrated internal control monitors DNA quality and detects potential PCR inhibition, ensuring confidence in diagnostic results. With optimized assay chemistry and compatibility with widely used real-time PCR platforms, this kit is ideal for clinical diagnostics and research focused on women’s health and microbiome-related conditions.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
The Gardnerella vaginalis (G. vaginalis) PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the qualitative detection of G. vaginalis DNA in human clinical samples. Based on advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly specific and reliable identification of this key bacterium associated with bacterial vaginosis (BV).
Targeting conserved regions of the G. vaginalis genome, including virulence-associated genes, the kit enables accurate detection even in low-copy samples. An integrated internal control monitors DNA quality and detects potential PCR inhibition, ensuring confidence in diagnostic results.
With optimized assay chemistry and compatibility with widely used real-time PCR platforms, this kit is ideal for clinical diagnostics and research focused on women’s health and microbiome-related conditions.
- High sensitivity detection (~250 IU/ml)
- Dual-channel FAM/HEX with internal control
- Ready-to-use, contamination-controlled workflow
- High specificity for G. vaginalis DNA
Qualitative PCR Detection of Gardnerella vaginalis
The G. vaginalis PCR Detection Kit is an in-vitro diagnostic real-time PCR assay developed for the qualitative identification of G. vaginalis DNA in human samples. Utilizing hydrolysis probe-based detection, the assay delivers high sensitivity and specificity for this clinically relevant bacterium.
The kit includes a ready-to-use master mix containing primers and probes for both G. vaginalis detection and an internal control gene, enabling simultaneous validation of sample quality and amplification efficiency.
Molecular Background
Gardnerella vaginalis is a facultatively anaerobic, Gram-variable bacterium commonly associated with bacterial vaginosis, a condition characterized by disruption of the normal vaginal microbiota. Overgrowth of G. vaginalis leads to a reduction in protective Lactobacillus species and an increase in vaginal pH.
This imbalance may result in symptoms such as abnormal discharge and odor, although infections can also be asymptomatic. G. vaginalis contributes to biofilm formation on the vaginal epithelium, which increases resistance to treatment and contributes to recurrence.
Accurate molecular detection is important for diagnosis, treatment monitoring, and understanding microbiome-related conditions.
Assay Performance & Utility
The G. vaginalis PCR Detection Kit is designed for reliable performance in routine diagnostic workflows. The assay uses dual-channel fluorescence detection: the FAM channel detects G. vaginalis DNA, while the HEX channel serves as an internal control to verify DNA quality and detect PCR inhibition.
The assay incorporates “hot start” PCR technology to minimize non-specific amplification and enhance sensitivity. Uracil-DNA glycosylase (UDG) is included to prevent carry-over contamination, ensuring consistent and reproducible results.
With high analytical sensitivity and near 100% specificity under validated conditions, the kit enables accurate detection across a range of sample types, particularly vaginal and cervical swabs.
Technical Specifications
- Assay Type: Qualitative Real-Time PCR (qPCR)
- Target: Gardnerella vaginalis DNA
- Detection Channels: FAM (G. vaginalis), HEX (Internal Control)
- Sensitivity: ~250 IU/ml (validated conditions)
- Sample Types: Vaginal swabs, cervical swabs, and other validated samples
- Input Material: Extracted DNA
- Compatible Instruments: ABI 7500, QuantStudio 3/5/7, Bio-Rad CFX, Rotor-Gene Q, LightCycler 480, and equivalent systems
- G. vaginalis qPCR Master Mix
- G. vaginalis Positive Control
- Nuclease-free Water
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Gardnerella vaginalis PCR Kit.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
