X
- No products in the list
The Hemochromatosis PCR Kit is a molecular biology assay designed for the analysis of genetic variation in the HFE gene. It enables the detection van key variants associated with iron metabolism using real-time PCR technology.
The kit supports multiplex analysis of multiple targets within a single workflow and is compatible with standard real-time PCR platforms, ensuring reliable and reproducible performance in molecular biology applications.
Note that this kit is for Research use Only (RUO).
The Hemochromatosis PCR Kit is designed for the analysis of genetic variation in the HFE gene using real-time PCR technology. It enables the detection of multiple variants—c.845G>A (p.Cys282Tyr), c.187C>G (p.His63Asp), and c.193A>T (p.Ser65Cys)—within a multiplex assay format, supporting efficient and comprehensive genetic analysis.
The HFE gene plays a key role in the regulation of iron metabolism. It is involved in the control of hepcidin expression, a hormone responsible for maintaining systemic iron balance. Under normal conditions, this regulatory mechanism ensures that iron absorption and storage remain within physiological limits.
Genetic variation in the HFE gene can affect this regulatory pathway. Variants such as C282Y, H63D, and S65C are widely studied in molecular biology due to their association with altered iron metabolism. These changes may influence the interaction between the HFE protein and other components involved in iron homeostasis, making the gene an important target for genetic analysis.
The Hemochromatosis PCR Kit is optimized for integration into standard laboratory workflows. Its multiplex design allows for the simultaneous amplification and detection of multiple target sequences, reducing the need for separate reactions and improving overall efficiency.
The assay uses fluorescent probe-based detection to ensure high specificity and reliable discrimination between target variants. This approach supports accurate identification of genetic variation while minimizing non-specific signals.
Genomic DNA extracted from peripheral blood is used as input material, with a recommended quantity of 150 ng per reaction. The workflow is designed to be straightforward, allowing laboratories to process samples efficiently while maintaining consistent performance.
Compatibility with widely used real-time PCR platforms enables easy implementation without the need for specialized equipment. This flexibility makes the kit suitable for a variety of laboratory environments and research applications.
The assay is developed using validated components and optimized conditions to support reproducible results. Its multiplex design and probe-based detection contribute to robust performance across different runs and sample types.
The inclusion of multiple targets within a single assay enhances efficiency while maintaining analytical clarity. This makes the kit a practical solution for laboratories requiring reliable analysis of HFE gene variants.
The Hemochromatosis PCR Kit provides a reliable and efficient solution for the analysis of genetic variation in the HFE gene. Its multiplex design, combined with real-time PCR technology and compatibility with standard laboratory equipment, supports consistent and reproducible performance.
With its streamlined workflow and comprehensive detection capabilities, the kit is well suited for molecular biology applications focused on iron metabolism and related genetic studies.
The kit contents the necessary reagents to perform 48 reactions. The reagents included in the kit are the following:
Instructions for Use
MSDS Hemochromatosis PCR kit
For any missing information or if you require additional details, please do not hesitate to contact us.