Hereditary Cancer NGS Assay
The Viennalab Hereditary Cancer NGS Assay is a library preparation kit for the analysis of germline variants in 31 cancer-associated genes by next-generation sequencing. The whole coding sequence of each gene is covered, a target region of 97 kb. Research use only. The panel includes BRCA1, BRCA2, the mismatch repair genes MLH1, MSH2, MSH6, PMS2 and EPCAM, and further genes associated with hereditary breast, ovarian, colorectal, pancreatic and skin cancer. One kit covers 16 reactions.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
- 31 cancer-associated genes, whole coding sequence, 97 kb target region
- Includes BRCA1, BRCA2, the mismatch repair genes and EPCAM
- Hybridisation capture library preparation with dual indexing
- DNA input 50 to 500 ng, and 150 to 500 ng for FFPE tissue
- Illumina sequencing, 2×150 bp paired end, GENOVESA analysis software
- 16 reactions per kit, REF 9-221
The Viennalab Hereditary Cancer NGS Assay prepares sequencing libraries for the analysis of germline variants in 31 genes associated with hereditary cancer. Research use only.
Genes covered
APC, ATM, BARD1, BLM, BMPR1A, BRCA1, BRCA2, BRIP1, CDH1, CDK4, CDKN2A, CHEK2, EPCAM, MLH1, MRE11A, MSH2, MSH6, MUTYH, NBN, PALB2, PMS2, PRSS1, PTEN, RAD50, RAD51C, RAD51D, SLX4, SMAD4, STK11, TP53 and VHL. The whole coding sequence of each gene is targeted, which comes to a region of 97 kb.
Method
Library preparation runs from enzymatic fragmentation and end repair with A-tailing, through adapter ligation and dual-index PCR, to targeted enrichment with biotinylated capture probes. The average fragment size after fragmentation is 350 to 450 bp, and the finished library shows a single peak between 200 and 800 bp with its maximum around 350 to 450 bp.
Requirements
The DNA input is 50 to 500 ng, and for DNA from formalin-fixed paraffin-embedded tissue 150 to 500 ng where possible. Sequencing is done on an Illumina platform with 2×150 bp paired-end reads; 0.8 to 1.2 million reads per sample give an average coverage of 100x with at least 95 per cent of all regions at 50x. Analysis runs in the GENOVESA software. DNA extraction reagents are not supplied, and the kit contents are listed without fill volumes.
Regulatory status
This kit is for research use only.
Library Preparation Box #1
- FEAT Buffer
- FEAT Enzyme
- Ligation Buffer
- Ligation Enzyme
- Adapter
- Activation Enzyme
- 2x PCR Mix
Library Preparation Box #2
- Dual Index Primer i5 (4 different primers)
- Dual Index Primer i7 (4 different primers)
Target Capture Box #1
- Block #1
- Block #2
- Block #3
- Capture Probe #2
- Post Capture PCR Forward Primer
- Post Capture PCR Reverse Primer
Target Capture Box #2
- Wash Buffer #1
- Wash Buffer #2
- Wash Buffer #3
- Library Hyb Buffer
Somatic Mutations Target Capture Box #3
- Somatic Mutations Capture Probe #1
Additional Provided Reagents – Beads
- Clean up Beads
- Streptavidin Beads
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
Brochure NGS Assays
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Hereditary Cancer NGS Assay.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
