Microblot-Array ANA
The Microblot-Array assay is intended for the diagnosis of systemic autoimmune diseases using IgG antibodies to extractable nuclear antigens in human serum or plasma in the general population. The qualitative, semi-quantitative and quantitative automated assay is designed for professional use in a laboratory.
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This kit in detail
Description
- Used antigen:
- Group 1 (ANA): Jo-1, PL-7, PL-12, EJ, OJ, KS, YARS, ZoA, ZoB, SAE-1, SAE-2, SRP54, Mi-2, TIF1γ, MDA5, NXP2, PMScl 75, PMScl 100, CENP A, CENP B, Scl70, POLR3A, NOR90, PDGFR-β, Fibrillarin, Th/To, Ro52, Ro60, La, SmB, SmD, RNP A, RNP C, RNP 68/70, P0, Ku, Nucleolin, dsDNA, Histone, Nucleosome, PCNA; Supplementary antigens: M2, DFS70
- Group 2 (Myositis): Jo-1, PL-7, PL-12, EJ, OJ, KS, YARS, ZoA, ZoB, SAE-1, SAE-2, SRP54, Mi-2, TIF1γ, MDA5, NXP2; Supplementary antigens: Ro52, PMScl 75, PMScl 100, Ku
- Group 3 (Scleroderma): CENP A, CENP B, Scl70, POLR3A, NOR90, PDGFR-β, Fibrillarin, Th/To, PMScl 75, PMScl 100, RNP A, RNP C, RNP 68/70; Supplementary antigens: Ro52, Ku, M2
- Group 4 (SLE and other connective tissue diseases): dsDNA, Histone, Nucleosome, PCNA, SmB, SmD, RNP A, RNP C, RNP 68/70, P0, Ku, Nucleolin, Ro52, Ro60, La, NOR90
- Antigens are applied in the form of microdots (spots) in triplets to a nitrocellulose membrane in a microtiter well format
- If specific antibodies are present in the sample, they will bind to the respective separate antigens
- The complex is labelled with Conjugate and detected through a colour reaction with Substrate (BCIP/NBT)
- The kit enables 96 tests
- Short incubation periods, total assay time: approximately 1.5 h.
- Each individual test includes control spots, which serves to check functionality and sensitivity of the sets, and also the spots for checking the presence of the conjugate
- Included positive controls enable validation of the tests
- For quantitative evaluation the wells contain calibration spots
- All reagents are supplied ready to use
- The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities)
- Evaluation using Microblot-Array reader and the appropriate software
Application
- Confirmation for ELIA or other screening tests
- Identification of individual antibodies types, differentiation of individual systemic autoimmune diseases
Brief assay procedure
- Dilute samples of serum/plasma (1:51)
- Pipette diluted samples
- Incubate at room temperature for 30 min.
- Wash 3 times for 5 min.
- Add Conjugate
- Incubate at room temperature for 30 min.
- Wash 3 times for 5 min.
- Add Substrate (BCIP/NPT)
- Incubate at room temperature for 15 min.
- Wash 2 times for 5 min. in distilled water
- Dry and evaluate test
Specifications
- EAN Code: 8595635308079
- Catalog number: ANAMA96
- Package size: 96 tests
- Regulatory status: IVD CE
- Storage: 2-8 °C
- Producer: TestLine Clinical Diagnostics s.r.o.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Microblot-Array ANA.
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