Microblot-Array Liver profile

Description

• Used antigen: LKM-1, LC-1, SLA/LP, Sp100, gp210, ASGPR, PML, Nup62, M2, 3E(BPO), OGDC-E2, PDC-E2 and Ro52.
• Antigens are applied in the form of microdots (spots) in triplets to a nitrocellulose membrane in a microtiter well format.
• If specific antibodies are present in the sample, they will bind to the respective separate antigens.
• The complex is labelled with Conjugate and detected through a colour reaction with Substrate (BCIP/NBT).
• The kit enables 48 tests.
• Short incubation periods, total assay time: approximately 1.5 h.
• Each individual test includes control spots, which serves to check functionality of the kits, and also the spots for checking the presence of the conjugate.
• Included positive controls enable validation of the tests.
• For quantitative evaluation the wells contain calibration spots.
• All reagents are supplied ready to use.
• The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities).
• Evaluation using Microblot-Array reader and the appropriate software.

Application

• Confirmation for ELIA or other screening tests.
• Identification of individual antibody types, differentiation of individual autoimmune liver diseases.

Brief assay procedure

1. Dilute samples of serum/plasma (1:51)
2. Pipette diluted samples
3. Incubate at room temperature for 30 min.
4. Wash 3 times for 5 min.
5. Add Conjugate
6. Incubate at room temperature for 30 min.
7. Wash 3 times for 5 min.
8. Add Substrate (BCIP/NPT)
9. Incubate at room temperature for 15 min.
10. Wash 2 times for 5 min. in distilled water
11. Dry and evaluate test

Specifications

• EAN Code: 8595635311697
• Catalog number: LKMMA48
• Package size: 48 tests
• Regulatory status: IVD CE2265
• Storage: 2-8 °C
• Producer: TestLine Clinical Diagnostics s.r.o.