MTHFR Polymorphism Kit

MTHFR Polymorphism Kit: Advanced Genetic Analysis

In modern molecular biology, the analysis of gene polymorphisms provides valuable insights into genetic variation. The imegen® MTHFR Polymorphism Kit is designed for the detection of both the c.677C>T (NM_005957.4: c.665C>T) variant and the wild-type allele of the MTHFR gene within a single real-time PCR assay.

Background

The MTHFR gene, located on chromosome 1p36.22, encodes the enzyme methylenetetrahydrofolate reductase, which plays a role in folate metabolism and the remethylation of homocysteine to methionine. The c.677C>T polymorphism is associated with reduced enzymatic activity and is widely studied in genetic research related to metabolic and biochemical pathways.

Key Features of the Kit

• Targeted Detection: Enables identification of both the c.677C>T variant and the corresponding wild-type sequence.
• Real-time PCR Technology: Utilizes sequence-specific oligonucleotides and fluorescent hydrolysis probes for accurate and sensitive detection.
• Positive Control: Includes a synthetic control representing a heterozygous genotype to support assay performance and result verification.
• Workflow Compatibility: Designed for use on standard real-time PCR platforms and compatible with commonly used laboratory protocols.
• Efficient Processing: Requires a small amount of genomic DNA (50 ng) from peripheral blood, with a streamlined workflow for rapid analysis.

Conclusion

The imegen®MTHFR Polymorphism Kit offers a reliable and efficient solution for the analysis of genetic variation within the MTHFR gene. Its robust design and compatibility with standard laboratory equipment support consistent performance in molecular biology workflows.