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The Mumps Virus (MuV) PCR Detection Kit is a high-performance real-time RT-qPCR assay designed for the qualitative detection of mumps virus RNA in human samples. Utilizing advanced hydrolysis probe (TaqMan®) technology, the assay enables highly sensitive and specific identification of MuV across clinically relevant specimen types.
Targeting conserved regions of the viral genome, the kit ensures reliable detection of circulating mumps virus strains. An integrated internal control system supports validation of RNA quality and detection of potential PCR inhibition, ensuring accurate and reproducible results.
With optimized one-step RT-qPCR chemistry, hot start technology, and compatibility with standard real-time PCR platforms, this kit provides a fast and dependable solution for clinical diagnostics and outbreak monitoring.
The MuV PCR Detection Kit is an in-vitro diagnostic real-time RT-qPCR assay developed for the qualitative identification of mumps virus RNA. The assay utilizes hydrolysis probe technology to amplify and detect specific viral targets with high sensitivity and specificity.
The kit includes a ready-to-use master mix with primers and probes for MuV detection and an internal control, enabling simultaneous verification of sample quality and amplification efficiency.
Mumps virus (MuV) is a highly neurotropic RNA virus responsible for mumps, an infectious disease characterized by inflammation of the salivary glands (parotitis). While many infections are mild or asymptomatic, symptomatic cases may present with fever, headache, malaise, and swelling of the parotid glands.
Complications can include orchitis, meningitis, encephalitis, hearing loss, and other neurological manifestations. Due to overlapping symptoms with other viral infections, accurate molecular detection is essential for diagnosis and public health control.
The assay is designed for rapid and reliable detection of MuV RNA in clinical samples such as buccal swabs, blood, plasma, serum, and lesion exudates. One-step RT-qPCR allows reverse transcription and amplification in a single reaction, improving workflow efficiency.
Hot start PCR technology reduces non-specific amplification, while uracil-DNA glycosylase (UDG) minimizes carry-over contamination. Dual-channel detection enables clear interpretation: the target-specific signal indicates MuV presence, while the internal control confirms RNA integrity and assay performance.
Instructions for Use