RAA End-Point Detection
The RAA End-Point Nucleic Acid Amplification Kit enables fast and highly sensitive nucleic acid amplification with end-point detection via agarose gel electrophoresis. Utilizing recombinase-aided amplification technology, the system performs isothermal amplification at a constant temperature, allowing rapid detection without the need for conventional PCR thermocycling. Developed for professional laboratory use, the kit combines optimized amplification reagents with a robust end-point detection workflow to produce clear and interpretable results. Supporting both DNA and RNA targets through RAA and RT-RAA configurations, the RAA end-point kit offers a streamlined approach for nucleic acid amplification, molecular assay development, and research-based detection workflows.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
The RAA End-Point Nucleic Acid Amplification Kit enables fast and highly sensitive nucleic acid amplification with end-point detection via agarose gel electrophoresis. Utilizing recombinase-aided amplification technology, the system performs isothermal amplification at a constant temperature, allowing rapid detection without the need for conventional PCR thermocycling.
Developed for professional laboratory use, the kit combines optimized amplification reagents with a robust end-point detection workflow to produce clear and interpretable results. Supporting both DNA and RNA targets through RAA and RT-RAA configurations, the RAA end-point kit offers a streamlined approach for nucleic acid amplification, molecular assay development, and research-based detection workflows.
- Rapid isothermal nucleic acid amplification
- End-point detection via agarose gel electrophoresis
- Supports DNA (RAA) and RNA targets (RT-RAA)
- Reliable results within 20–40 minute
RAA End-Point Nucleic Acid Amplification
The RAA End-Point Nucleic Acid Amplification system is developed for fast and sensitive nucleic acid amplification with detection based on agarose gel electrophoresis. Designed for professional laboratory use, the kit amplifies DNA or RNA targets using recombinase-aided amplification (RAA), an isothermal technology that mimics natural cellular processes to synthesize double-stranded DNA at a constant temperature.
Operating at temperatures between approximately 37–42 °C, the system enables rapid nucleic acid amplification without the need for thermal cycling. Following amplification, the resulting products are analyzed using agarose gel electrophoresis, allowing visualization of amplified fragments and confirmation of target-specific amplification. This approach enables sensitive and reliable detection while maintaining a controlled and well-established molecular workflow.
The kit includes lyophilized amplification tubes containing the enzyme mix, together with essential reaction components optimized for efficient amplification and downstream analysis.
Kit Components
The RAA End-Point Nucleic Acid Amplification kit typically includes the following components:
- Lyophilized amplification tubes containing enzyme mix
- Buffer V
- Magnesium acetate I
- Positive control material
- Forward primer for positive control
- Reverse primer for positive control
- Purified water
- Instruction manual
Note: Reagents and equipment for agarose gel electrophoresis are not included and must be supplied separately.
Supported Targets
The kit supports nucleic acid amplification of:
- DNA targets (RAA)
- RNA targets (RT-RAA format)
- Synthetic control templates
- Extracted nucleic acids from biological samples
Compatible Applications
Typical workflows include:
- Molecular assay development
- Rapid nucleic acid screening
- Research-based pathogen detection
- Validation of custom primer sets
Simple and Structured Protocol
RT-RAA and RAA End-point detection workflow
- Prepare mastermix containing Buffer V, primers, and purified water
- Dispense mastermix into amplification tubes containing the lyophilized enzyme mix
- Add magnesium acetate to the tube lid and introduce nucleic acid template
- Close tubes, mix gently, and briefly centrifuge
- Incubate at approximately 37–42 °C using a heating block or water bath
- Perform amplification for approximately 20–40 minutes
- After amplification, extract reaction products and analyze using agarose gel electrophoresis
The amplification process typically generates detectable products within 20–40 minutes, allowing rapid nucleic acid detection for research and assay development workflows.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Frequently paired with this kit
Other assays and instruments lab teams add alongside the RAA End-Point Detection.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.