RAA End-Point Detection

RAA End-Point Nucleic Acid Amplification

The RAA End-Point Nucleic Acid Amplification system is developed for fast and sensitive nucleic acid amplification with detection based on agarose gel electrophoresis. Designed for professional laboratory use, the kit amplifies DNA or RNA targets using recombinase-aided amplification (RAA), an isothermal technology that mimics natural cellular processes to synthesize double-stranded DNA at a constant temperature.

Operating at temperatures between approximately 37–42 °C, the system enables rapid nucleic acid amplification without the need for thermal cycling. Following amplification, the resulting products are analyzed using agarose gel electrophoresis, allowing visualization of amplified fragments and confirmation of target-specific amplification. This approach enables sensitive and reliable detection while maintaining a controlled and well-established molecular workflow.

The kit includes lyophilized amplification tubes containing the enzyme mix, together with essential reaction components optimized for efficient amplification and downstream analysis.

Kit Components

The RAA End-Point Nucleic Acid Amplification kit typically includes the following components:

• Lyophilized amplification tubes containing enzyme mix
• Buffer V
• Magnesium acetate I
• Positive control material
• Forward primer for positive control
• Reverse primer for positive control
• Purified water
• Instruction manual

Note: Reagents and equipment for agarose gel electrophoresis are not included and must be supplied separately.

Supported Targets

The kit supports nucleic acid amplification of:

• DNA targets (RAA)
• RNA targets (RT-RAA format)
• Synthetic control templates
• Extracted nucleic acids from biological samples

Compatible Applications

Typical workflows include:

• Molecular assay development
• Rapid nucleic acid screening
• Research-based pathogen detection
• Validation of custom primer sets

Simple and Structured Protocol

RT-RAA and RAA End-point detection workflow

• Prepare mastermix containing Buffer V, primers, and purified water
• Dispense mastermix into amplification tubes containing the lyophilized enzyme mix
• Add magnesium acetate to the tube lid and introduce nucleic acid template
• Close tubes, mix gently, and briefly centrifuge
• Incubate at approximately 37–42 °C using a heating block or water bath
• Perform amplification for approximately 20–40 minutes
• After amplification, extract reaction products and analyze using agarose gel electrophoresis

The amplification process typically generates detectable products within 20–40 minutes, allowing rapid nucleic acid detection for research and assay development workflows.