RAA Lateral Flow Detection Kit

RAA Lateral Flow Nucleic Acid Amplification

The RAA Lateral Flow Nucleic Acid Amplification system is developed for fast and sensitive nucleic acid amplification with visual detection using lateral flow strips. Designed for professional laboratory use, the kit amplifies DNA or RNA targets using recombinase-aided amplification (RAA), an isothermal technology that mimics natural cellular processes to synthesize double-stranded DNA at a constant temperature.

Operating at temperatures between approximately 37–42 °C, the system enables rapid nucleic acid amplification without the need for thermal cycling. When combined with labeled primers and probes, the amplification products can be detected using a lateral flow strip that generates visible lines indicating the presence of the target sequence. This approach enables highly sensitive detection while maintaining a simplified molecular workflow.

The kit includes lyophilized amplification tubes containing the enzyme mix, together with essential reaction components optimized for efficient amplification and lateral flow detection.

Kit Components

The RAA Lateral Flow Nucleic Acid Amplification kit typically includes the following components:

-Lyophilized amplification tubes containing enzyme mix
-Buffer VI
-Magnesium acetate I
-Positive control material
-Forward primer for positive control
-Reverse primer for positive control
-Lateral flow probe for positive control
-Purified water
-Instruction manual
Note: Lateral flow test strips are not included and must be supplied separately.

Supported Targets

The kit supports nucleic acid amplification of:

• DNA targets
• RNA targets (when using RT-RAA variants)
• Synthetic control templates
• Extracted nucleic acids from biological samples

Compatible Applications

Typical workflows include:

• Molecular assay development
• Rapid nucleic acid screening
• Research-based pathogen detection
• Validation of custom primer and probe sets

Simple and Streamlined Protocol

Lateral flow detection workflow

1. prepare mastermix of reagents buffer, magnesium acetate and primer-probe mix
2. open tube with freeze-dried reagents and add mastermix into the inner surface of the tube lid
3. add extracted nucleic acids to the lid as well
4. close tube, short-spin and mix by pulse-vortexing
5. place tube in your heating block or instrument
6. incubate reactions for 20 minutes (at approx. 37-42°C)
7. open the tube and dilute the amplification mix 1:10 with PBS
8. add the diluted amplified nucleic acids to your detection lateral-flow strip

The amplification process typically generates detectable results within 15–30 minutes, allowing rapid nucleic acid detection for research and assay development workflows.