St. Louis Encephalitis Virus (SLEV) PCR Kit
The St. Louis Encephalitis Virus (SLEV) PCR Detection Kit is a high-performance real-time RT-qPCR assay designed for the qualitative detection of SLEV RNA in human samples. Utilizing advanced hydrolysis probe (TaqMan®) technology, the assay ensures highly sensitive and specific identification of this mosquito-borne flavivirus. Targeting conserved regions of the SLEV genome, the kit enables reliable detection across circulating viral strains. An integrated internal control system verifies RNA quality and detects potential PCR inhibition, ensuring accurate and reproducible results. With optimized one-step RT-qPCR chemistry and compatibility with standard real-time PCR platforms, this kit provides an efficient solution for clinical diagnostics and surveillance of arboviral infections.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
- High sensitivity detection down to 10 copies per reaction
- High specificity for accurate SLEV RNA identification
- Dual-channel detection with internal control (FAM/HEX)
- Ready-to-use one-step RT-qPCR workflow
Qualitative RT-qPCR Detection of SLEV RNA
The SLEV PCR Detection Kit is an in-vitro diagnostic real-time RT-qPCR assay developed for the qualitative identification of St. Louis encephalitis virus RNA. The assay utilizes hydrolysis probe technology to amplify and detect specific viral targets with high sensitivity and specificity.
The kit includes a ready-to-use master mix containing primers and probes for SLEV detection and an internal control, enabling simultaneous verification of sample quality and amplification efficiency.
Molecular Background
St. Louis encephalitis virus (SLEV) is a mosquito-borne flavivirus transmitted primarily through infected mosquitoes, with birds acting as natural reservoir hosts. Human infection is typically incidental.
Most infections are asymptomatic or mild, presenting with flu-like symptoms such as fever, headache, and fatigue. However, severe cases can result in encephalitis, particularly in elderly or immunocompromised individuals, with symptoms including high fever, neck stiffness, confusion, seizures, and potentially coma or death.
Assay Performance & Utility
The assay is designed for rapid and reliable detection of SLEV RNA in clinical samples such as blood, serum, plasma, and cerebrospinal fluid (CSF). One-step RT-qPCR enables reverse transcription and amplification in a single reaction, improving workflow efficiency.
Hydrolysis probe-based detection ensures high specificity, while optimized assay chemistry provides robust performance even in low viral load samples. The internal control system supports confident interpretation of both positive and negative results.
Technical Specifications
- Assay Type: Qualitative Real-Time RT-qPCR
- Target: St. Louis Encephalitis Virus (SLEV) RNA
- Detection Channels: FAM (SLEV), HEX (Internal Control)
- Sensitivity: ≥10 copies per reaction
- Sample Types: Blood, serum, plasma, cerebrospinal fluid (CSF)
- Input Material: Extracted RNA
- Technology: One-step RT-qPCR with hydrolysis probe detection
- Compatible Instruments: ABI 7500, QuantStudio series, Bio-Rad CFX, Rotor-Gene Q, LightCycler 480, and equivalent systems
- qPCR Master Mix
- Positive control
- Nuclease-free water
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Documentation available on request, contact us and we will send it over.
Frequently paired with this kit
Other assays and instruments lab teams add alongside the St. Louis Encephalitis Virus (SLEV) PCR Kit.
iCassette - Mycobacterium Tuberculosis and Rifampicin resistance
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
