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The St. Louis Encephalitis Virus (SLEV) PCR Detection Kit is a high-performance real-time RT-qPCR assay designed for the qualitative detection of SLEV RNA in human samples. Utilizing advanced hydrolysis probe (TaqMan®) technology, the assay ensures highly sensitive and specific identification of this mosquito-borne flavivirus.
Targeting conserved regions of the SLEV genome, the kit enables reliable detection across circulating viral strains. An integrated internal control system verifies RNA quality and detects potential PCR inhibition, ensuring accurate and reproducible results.
With optimized one-step RT-qPCR chemistry and compatibility with standard real-time PCR platforms, this kit provides an efficient solution for clinical diagnostics and surveillance of arboviral infections.
The SLEV PCR Detection Kit is an in-vitro diagnostic real-time RT-qPCR assay developed for the qualitative identification of St. Louis encephalitis virus RNA. The assay utilizes hydrolysis probe technology to amplify and detect specific viral targets with high sensitivity and specificity.
The kit includes a ready-to-use master mix containing primers and probes for SLEV detection and an internal control, enabling simultaneous verification of sample quality and amplification efficiency.
St. Louis encephalitis virus (SLEV) is a mosquito-borne flavivirus transmitted primarily through infected mosquitoes, with birds acting as natural reservoir hosts. Human infection is typically incidental.
Most infections are asymptomatic or mild, presenting with flu-like symptoms such as fever, headache, and fatigue. However, severe cases can result in encephalitis, particularly in elderly or immunocompromised individuals, with symptoms including high fever, neck stiffness, confusion, seizures, and potentially coma or death.
The assay is designed for rapid and reliable detection of SLEV RNA in clinical samples such as blood, serum, plasma, and cerebrospinal fluid (CSF). One-step RT-qPCR enables reverse transcription and amplification in a single reaction, improving workflow efficiency.
Hydrolysis probe-based detection ensures high specificity, while optimized assay chemistry provides robust performance even in low viral load samples. The internal control system supports confident interpretation of both positive and negative results.
Instructions for Use