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TORCH Panel Kit
The TORCH Panel from TRUPCR® is a highly sensitive real-time PCR assay for the simultaneous detection and differentiation of Toxoplasma gondii, Rubella virus, Cytomegalovirus (CMV), and Herpes simplex virus types 1 and 2 from clinical samples. The assay uses two multiplexed tubes with three dye channels per tube, enabling fast and reliable detection in a single-step PCR format. This TORCH Panel incorporates hot-start technology to reduce nonspecific amplification and includes an endogenous internal control to validate sample quality and rule out PCR inhibition. Designed for compatibility with major real-time PCR platforms, it is a valuable tool for prenatal screening, transplant medicine, and infectious disease diagnostics. The TRUPCR® TORCH Panel is optimized for accuracy, speed, and workflow efficiency in high-throughput laboratory settings.
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This kit in detail
The TORCH Panel from TRUPCR® is a highly sensitive real-time PCR assay for the simultaneous detection and differentiation of Toxoplasma gondii, Rubella virus, Cytomegalovirus (CMV), and Herpes simplex virus types 1 and 2 from clinical samples. The assay uses two multiplexed tubes with three dye channels per tube, enabling fast and reliable detection in a single-step PCR format. This TORCH Panel incorporates hot-start technology to reduce nonspecific amplification and includes an endogenous internal control to validate sample quality and rule out PCR inhibition. Designed for compatibility with major real-time PCR platforms, it is a valuable tool for prenatal screening, transplant medicine, and infectious disease diagnostics. The TRUPCR® TORCH Panel is optimized for accuracy, speed, and workflow efficiency in high-throughput laboratory settings.
- Detects all key TORCH Panel pathogens in one run
- Built-in internal control ensures reliable results
- Hot-start PCR for high sensitivity and specificity
- Single-step workflow, compatible with most PCR instruments
What is in the TORCH Panel?
The TORCH Panel is a molecular diagnostic test designed to detect four pathogens responsible for serious congenital and perinatal infections. It includes:
Tube 1
- Cytomegalovirus (CMV) – FAM/Green
- Toxoplasma gondii – HEX/Yellow
- Rubella virus – ROX/Orange
Tube 2
- Herpes Simplex Virus 1 – FAM/Green
- Herpes Simplex Virus 2 – HEX/Yellow
- Endogenous Internal Control – ROX/Orange
These pathogens are detected using real-time PCR in a multiplex format, allowing simultaneous amplification of three targets per tube. The assay provides rapid, accurate diagnosis, which is crucial in prenatal and neonatal care.
Role of Different Targets in Disease
Toxoplasma gondii
An intracellular parasite transmitted via contaminated food, water, or vertically during pregnancy. While often asymptomatic in immunocompetent adults, congenital toxoplasmosis can lead to miscarriage, brain damage, or vision loss in infants. India’s prevalence ranges from 20–37%, with higher rates in South India.
Rubella Virus
A mild viral illness in children and adults but potentially devastating during pregnancy. Rubella infection in early pregnancy can cause congenital rubella syndrome (CRS), leading to birth defects like deafness, cataracts, and heart anomalies. Early detection and vaccination are key to prevention.
Cytomegalovirus (CMV)
A widespread herpesvirus that causes severe illness in immunocompromised patients and is the most common congenital viral infection. Infected newborns may experience developmental delays, hearing loss, or vision impairment. CMV may remain latent and reactivate, making detection critical in high-risk populations.
Herpes Simplex Virus 1 & 2 (HSV-1 & HSV-2)
HSV-1 is commonly associated with oral lesions; HSV-2 with genital infections. Both can be transmitted perinatally, leading to neonatal herpes, which may cause encephalitis, disseminated disease, or death. Many infections are asymptomatic, making molecular detection essential for diagnosis and prevention.
Detecting these pathogens together in a TORCH Panel is essential for early intervention, especially in pregnant individuals and immunocompromised patients.
About the TRUPCR® TORCH Panel Kit
The TRUPCR® TORCH Panel Kit is an in vitro nucleic acid amplification assay designed for clinical diagnostics, offering:
- Multiplex detection of all TORCH pathogens
- Endogenous internal control for RNA extraction and amplification integrity
- CDC-aligned assay design for reliable target amplification
- Hot-start polymerase for enhanced specificity
- Compatibility with multiple qPCR platforms
The TRUPCR® TORCH Panel is developed for use with clinical specimens including blood, amniotic fluid, and swabs. It is suitable for high-throughput labs requiring dependable, rapid screening for vertically transmitted infections.
Principle and Workflow
The TORCH Panel operates using a single-step real-time PCR approach:
Workflow Overview:
-
Sample Extraction
RNA or DNA is extracted from clinical material using standard nucleic acid purification kits. -
Multiplex Reaction Setup
Two reaction tubes are prepared per sample, each containing specific primers, probes, and hot-start polymerase enzymes. -
Real-Time Amplification
Each tube runs in a thermal cycler capable of detecting FAM, HEX, and ROX fluorophores. The internal control is monitored to validate assay performance. -
Result Interpretation
Cycle threshold (Ct) values are reviewed to determine target presence. The presence of a valid RNaseP or internal control signal ensures result reliability.
This method supports accurate, early diagnosis without manual post-PCR processing.
Clinical Application
The TORCH Panel is essential in:
- Prenatal screening for congenital infections
- Neonatal diagnostics in suspected congenital disease
- Fertility and reproductive health clinics
- Transplant and oncology departments screening immunocompromised patients
- General infectious disease diagnostics in hospitals and reference labs
Early detection of TORCH pathogens can reduce perinatal complications, guide treatment strategies, and support infection control programs. The TRUPCR® TORCH Panel provides labs with a robust and efficient diagnostic solution to improve patient care in maternal-fetal medicine and beyond.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Product Catalogus
Frequently paired with this kit
Other assays and instruments lab teams add alongside the TORCH Panel Kit.
iCassette - 18 High Risk Human Papillomavirus and HPV 6 & 11
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