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The Treponema pallidum (T. pallidum) PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the qualitative detection of T. pallidum DNA in human clinical samples. Based on advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly specific and reliable identification of the bacterium responsible for syphilis and related treponemal diseases.
Targeting conserved regions within the T. pallidum genome, the kit enables accurate detection even at low DNA concentrations. An integrated internal control is included to monitor DNA extraction quality and detect potential PCR inhibition, ensuring confidence in every result.
With a detection sensitivity down to 10 copies per reaction and compatibility with widely used real-time PCR platforms, this kit is an ideal solution for clinical diagnostics and infectious disease research laboratories requiring rapid and precise pathogen detection.
The T. pallidum PCR Detection Kit is an in-vitro diagnostic real-time PCR assay developed for the qualitative identification of T. pallidum DNA in human samples. Utilizing hydrolysis probe-based detection, the assay delivers high sensitivity and specificity for this clinically important sexually transmitted pathogen.
The kit includes a ready-to-use master mix containing primers and probes for both T. pallidum detection and an internal control gene, enabling simultaneous validation of sample quality and amplification efficiency.
Treponema pallidum is a Gram-negative, spiral-shaped bacterium responsible for syphilis, as well as related diseases such as bejel and yaws. Transmission primarily occurs through sexual contact via mucosal membranes or skin lesions, but vertical transmission from mother to fetus can also occur. Infection with T. pallidum increases susceptibility to other infections, including HIV.
Early and accurate molecular detection is essential for diagnosis, treatment monitoring, and prevention of disease transmission.
The T. pallidum PCR Detection Kit is designed for robust performance in routine diagnostic workflows. The assay uses dual-channel fluorescence detection: the FAM channel detects T. pallidum DNA, while the HEX channel serves as an internal control to verify DNA quality and identify potential PCR inhibition.
The assay incorporates “hot start” PCR technology to minimize non-specific amplification and enhance sensitivity. Additionally, uracil-DNA glycosylase (UDG) is included to prevent carry-over contamination, ensuring reliable and reproducible results.
With high analytical sensitivity and near 100% specificity under validated conditions, the kit enables accurate detection even in low-copy samples. It is suitable for a range of specimen types, including vaginal and urethral swabs and urine samples, making it a versatile solution for clinical laboratories.
Instructions for Use