Trichomonas vaginalis PCR Kit
The Trichomonas vaginalis (T. vaginalis) PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the qualitative detection of T. vaginalis DNA in human clinical samples. Utilizing advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly specific and reliable identification of this common sexually transmitted parasite. Targeting conserved regions of the T. vaginalis genome, the kit enables accurate detection even in low-copy samples. An integrated internal control monitors DNA quality and detects potential PCR inhibition, ensuring confidence in diagnostic results. With a detection sensitivity down to 10 copies per reaction and rapid turnaround time, this kit is ideal for clinical diagnostics and sexual health screening programs requiring fast and precise STI detection.
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This kit in detail
The Trichomonas vaginalis (T. vaginalis) PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the qualitative detection of T. vaginalis DNA in human clinical samples. Utilizing advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly specific and reliable identification of this common sexually transmitted parasite.
Targeting conserved regions of the T. vaginalis genome, the kit enables accurate detection even in low-copy samples. An integrated internal control monitors DNA quality and detects potential PCR inhibition, ensuring confidence in diagnostic results.
With a detection sensitivity down to 10 copies per reaction and rapid turnaround time, this kit is ideal for clinical diagnostics and sexual health screening programs requiring fast and precise STI detection.
- High sensitivity (≥10 copies/reaction)
- Dual-channel FAM/HEX with internal control
- Rapid, ready-to-use workflow (~40 min run time)
- High specificity for T. vaginalis DNA
Qualitative PCR Detection of Trichomonas vaginalis
The T. vaginalis PCR Detection Kit is an in-vitro diagnostic real-time PCR assay developed for the qualitative identification of T. vaginalis DNA in human samples. Utilizing hydrolysis probe-based detection, the assay delivers high sensitivity and specificity for this clinically significant sexually transmitted pathogen.
The kit includes a ready-to-use master mix containing primers and probes for both T. vaginalis detection and an internal control gene, enabling simultaneous validation of sample quality and amplification efficiency.
Molecular Background
Trichomonas vaginalis is a protozoan parasite responsible for trichomoniasis, one of the most common non-viral sexually transmitted infections worldwide. Infection can affect both men and women, although it is often asymptomatic in men.
In women, symptoms may include vaginal discharge, irritation, and discomfort during urination or intercourse. Untreated infections can lead to complications such as increased susceptibility to other STIs and adverse pregnancy outcomes.
Accurate molecular detection is essential for diagnosis, treatment, and prevention of transmission.
Assay Performance & Utility
The T. vaginalis PCR Detection Kit is designed for reliable performance in routine diagnostic workflows. The assay uses dual-channel fluorescence detection: the FAM channel detects T. vaginalis DNA, while the HEX channel serves as an internal control to verify DNA quality and detect PCR inhibition.
The assay incorporates “hot start” PCR technology to minimize non-specific amplification and enhance sensitivity. Uracil-DNA glycosylase (UDG) is included to prevent carry-over contamination, ensuring consistent and reproducible results.
With high analytical sensitivity and near 100% specificity under validated conditions, the kit enables accurate detection across a range of specimen types, including vaginal, cervical, and urethral swabs, as well as urine samples.
Technical Specifications
- Assay Type: Qualitative Real-Time PCR (qPCR)
- Target: Trichomonas vaginalis DNA
- Detection Channels: FAM (T. vaginalis), HEX (Internal Control)
- Sensitivity: ≥10 copies per reaction
- Sample Types: Vaginal swabs, cervical swabs, urethral swabs, urine samples
- Input Material: Extracted DNA
- Compatible Instruments: ABI 7500, QuantStudio 3/5/7, Bio-Rad CFX, Rotor-Gene Q, LightCycler 480, and equivalent systems
- T. vaginalis qPCR Master Mix
- T. vaginalis Positive Control
- Nuclease-free Water
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
Frequently paired with this kit
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