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The Ureaplasma parvum, Ureaplasma urealyticum and Mycoplasma hominis Multiplex PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the simultaneous qualitative detection of three clinically relevant urogenital pathogens in human samples. Based on advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly specific and reliable identification in a single multiplex reaction.
Targeting conserved regions within each pathogen’s genome, the kit enables accurate detection even in low-copy samples. An integrated internal control monitors DNA quality and detects potential PCR inhibition, ensuring confidence in diagnostic results.
With optimized multiplex design and compatibility with widely used real-time PCR platforms, this kit is ideal for clinical diagnostics and sexual health screening programs requiring comprehensive STI detection.
The multiplex PCR Detection Kit is an in-vitro diagnostic real-time PCR assay developed for the qualitative identification of Ureaplasma parvum, Ureaplasma urealyticum, and Mycoplasma hominis DNA in human samples. Utilizing hydrolysis probe-based multiplex detection, the assay delivers high sensitivity and specificity for multiple pathogens in a single reaction.
The kit includes a ready-to-use master mix containing primers and probes for each target organism, along with an internal control gene, enabling simultaneous validation of sample quality and amplification efficiency.
Ureaplasma parvum, Ureaplasma urealyticum, and Mycoplasma hominis are small, cell wall–deficient bacteria belonging to the Mycoplasmataceae family and commonly colonize the human urogenital tract.
Although often asymptomatic, these organisms can act as opportunistic pathogens and are associated with conditions such as non-gonococcal urethritis, bacterial vaginosis, pelvic inflammatory disease, infertility, and adverse pregnancy outcomes including preterm birth. Transmission occurs primarily through sexual contact or vertically during childbirth.
Due to their lack of a cell wall, these organisms are inherently resistant to certain antibiotics, making accurate and timely detection essential for appropriate treatment and patient management.
The multiplex PCR Detection Kit is designed for robust performance in routine diagnostic workflows. The assay uses multi-channel fluorescence detection to differentiate between pathogens:
The assay incorporates “hot start” PCR technology to minimize non-specific amplification and enhance sensitivity. Uracil-DNA glycosylase (UDG) is included to prevent carry-over contamination, ensuring consistent and reproducible results.
With multiplex capability and high analytical performance, the kit enables efficient detection of multiple urogenital pathogens in a single assay, reducing time and laboratory workload.
Instructions for Use