Yersinia Pestis PCR Kit
The Yersinia pestis (Y. pestis) PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the qualitative detection of Y. pestis DNA in human and animal samples. Utilizing advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly specific and reliable identification of the bacterium responsible for plague. Targeting conserved regions within the Y. pestis genome, including key virulence genes, the kit enables accurate detection even in low-copy samples. An integrated internal control monitors DNA quality and detects potential PCR inhibition, ensuring confidence in diagnostic results. With rapid turnaround and high analytical sensitivity, this kit is ideal for clinical diagnostics, outbreak response, and research laboratories requiring fast and accurate pathogen identification.
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This kit in detail
The Yersinia pestis (Y. pestis) PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the qualitative detection of Y. pestis DNA in human and animal samples. Utilizing advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly specific and reliable identification of the bacterium responsible for plague.
Targeting conserved regions within the Y. pestis genome, including key virulence genes, the kit enables accurate detection even in low-copy samples. An integrated internal control monitors DNA quality and detects potential PCR inhibition, ensuring confidence in diagnostic results.
With rapid turnaround and high analytical sensitivity, this kit is ideal for clinical diagnostics, outbreak response, and research laboratories requiring fast and accurate pathogen identification.
- Ultra-high sensitivity (~2.4 copies/µl)
- Dual-channel FAM/HEX with internal control
- Ready-to-use, contamination-controlled workflow
- High specificity for Y. pestis DNA
Qualitative PCR Detection of Yersinia pestis
The Y. pestis PCR Detection Kit is an in-vitro diagnostic real-time PCR assay developed for the qualitative identification of Y. pestis DNA in human and animal samples. Utilizing hydrolysis probe-based detection, the assay delivers high sensitivity and specificity for this clinically significant zoonotic pathogen.
The kit includes a ready-to-use master mix containing primers and probes for both Y. pestis detection and an internal control gene, enabling simultaneous validation of sample quality and amplification efficiency.
Molecular Background
Yersinia pestis is a Gram-negative bacterium and the causative agent of plague, including bubonic, septicemic, and pneumonic forms. It is a zoonotic pathogen typically transmitted via fleas from infected mammals, particularly rodents.
In addition to vector-borne transmission, pneumonic plague can spread via respiratory droplets, making rapid detection critical for infection control. Early diagnosis enables effective antibiotic treatment and significantly improves patient outcomes.
Assay Performance & Utility
The Y. pestis PCR Detection Kit is designed for reliable performance in routine diagnostic and emergency response settings. The assay uses dual-channel fluorescence detection: the FAM channel detects Y. pestis DNA, while the HEX channel serves as an internal control to verify DNA quality and detect PCR inhibition.
The assay incorporates “hot start” PCR technology to minimize non-specific amplification and enhance sensitivity. Uracil-DNA glycosylase (UDG) is included to prevent carry-over contamination, ensuring consistent and reproducible results.
With high analytical sensitivity (down to ~2.4 copies/µl under validated conditions) and near 100% specificity, the kit enables accurate detection even in low bacterial load samples. It is suitable for a range of specimen types, including plasma, serum, sputum, urine, and other validated samples.
Technical Specifications
- Assay Type: Qualitative Real-Time PCR (qPCR)
- Target: Yersinia pestis DNA (caf1, pla genes)
- Detection Channels: FAM (Y. pestis), HEX (Internal Control)
- Sensitivity: ~2.4 copies/µl (validated conditions)
- Sample Types: Plasma, serum, sputum, urine, feces, and other validated samples
- Input Material: Extracted DNA
- Compatible Instruments: ABI 7500, QuantStudio 3/5/7, Bio-Rad CFX, Rotor-Gene Q, LightCycler 480, and equivalent systems
- Y. pestis qPCR Master Mix
- Y. pestis Positive Control
- Nuclease-free Water
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
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