5-Gene-Multiplex 1% AF cfDNA in Plasma
The 5-Gene-Multiplex 1% AF cfDNA in Plasma from SensID is a reference material for liquid biopsy assays, used in both research and clinical diagnostics. It targets key mutations in five genes, AKT1, BRAF, KRAS, PIK3CA and ERBB2, which are associated with cancers such as lung, breast and colorectal cancer and melanoma. Containing a 1% allelic frequency of these mutations, the product is suited to digital PCR and next-generation sequencing (NGS). It acts as a positive control standard for validating and developing sequencing protocols and PCR methods, and for determining the detection limits of different diagnostic techniques. The mutations included are AKT1 (p.E17K); BRAF (p.V600E); KRAS (multiple variants); PIK3CA (p.E545K, p.H1047R); and ERBB2 (p.Y772_A775dup). These mutations are directly linked to specific treatments, such as vemurafenib for BRAF and cetuximab for KRAS mutations. The 5-Gene-Multiplex 1% AF cfDNA in Plasma supports accurate liquid biopsy testing and the development of cancer diagnostics.
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This kit in detail
- Comprehensive Mutation Detection: Includes critical mutations in AKT1, BRAF, KRAS, PIK3CA, and ERBB2.
- Reliable Positive Control: Serves as a standard for digital PCR and NGS.
- Ideal for Diagnostic Assays: Perfect for determining the detection limits and developing.
- Supports Targeted Therapies: Directly linked to therapeutic strategies.
The 5-Gene-Multiplex 1% AF cfDNA in Plasma product from SensID is a highly specialized and critical tool for liquid biopsy assays, primarily used in diagnostic and research applications. This product is designed to target five key mutations across essential genes: AKT1, BRAF, KRAS, PIK3CA, and ERBB2, mutations that are commonly linked to a range of cancers including lung, breast, colorectal, and melanoma. These mutations play an important role in cancer progression and are crucial for making informed therapeutic decisions.
The 5-Gene-Multiplex 1% AF cfDNA in Plasma contains a 1% allelic frequency (AF) of the targeted mutations. It serves as a positive control standard for techniques like digital PCR and next-generation sequencing (NGS). This tool is especially useful in validating and developing sequencing protocols, PCR methods, and assessing detection limits, which are crucial for achieving accurate and reliable results.
The specific mutations included in this product are:
- AKT1 (p.E17K),
- BRAF (p.V600E),
- KRAS (multiple variants),
- PIK3CA (p.E545K, p.H1047R),
- ERBB2 (p.Y772_A775dup).
These mutations are essential for targeted therapies. For example, BRAF mutations like p.V600E are treated with drugs such as vemurafenib, while KRAS mutations are addressed with therapies like cetuximab. Similarly, mutations in PIK3CA and AKT1 direct personalized treatments.
The 5-Gene-Multiplex 1% AF cfDNA in Plasma product is used in the development of cancer diagnostics, in particular in the rapidly evolving field of liquid biopsy testing. By providing a reliable and accurate benchmark, it helps facilitate the development of more precise and personalized cancer treatments, ensuring that assays used for liquid biopsy can detect genetic mutations with confidence and clarity.
5-Gene-Multiplex 1% AF cfDNA in Plasma
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Other assays and instruments lab teams add alongside the 5-Gene-Multiplex 1% AF cfDNA in Plasma.
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