Imegen-Alpha-1-AT

The imegen® Alpha-1-AT Kit: Advanced Genetic Analysis

Understanding genetic variation is essential in modern molecular biology. The imegen® Alpha-1-AT Kit (IMG-211) is a precise and reliable tool developed for the detection of specific mutations associated with alpha-1 antitrypsin deficiency. It targets the Glu342Lys (PI-Z; rs28929474; c.1096G>A) and Glu264Val (PI-S; rs17580; c.863A>T) variants within the SERPINA1 gene.

Key Features:

• Mutation Detection: Designed for accurate identification of PI-Z and PI-S variants within the SERPINA1 gene.
• Real-time PCR Technology: Utilizes real-time PCR for sensitive and specific DNA amplification and detection.
• High Sensitivity: Capable of detecting low levels of target DNA with consistent performance.
• Synthetic Controls: Includes synthetic positive controls representing heterozygous genotypes to support assay performance and verification.

Validation and Ease of Use:

The imegen® Alpha-1-AT Kit has been validated using characterized samples and synthetic DNA, demonstrating robust performance in mutation detection. The workflow requires 100 ng of genomic DNA from peripheral blood and is designed for straightforward integration into standard laboratory procedures. Clear instructions support efficient implementation.

Conclusion:

The imegen® Alpha-1-AT Kit provides a reliable solution for the analysis of genetic variants associated with alpha-1 antitrypsin deficiency. With its strong technical performance and compatibility with a wide range of real-time PCR platforms, it supports consistent and high-quality molecular biology workflows.