Cambridge II Syndrome qPCR kit
The imegen® Cambridge II kit is a molecular biology tool for the detection of the c.1246G>T (p.Ala416Ser) variant in the SERPINC1 gene. This variant is associated with Cambridge II syndrome, a hereditary condition related to thrombophilia. The kit utilizes real-time PCR technology to enable accurate detection of the target sequence and includes 48 reaction tubes. It is designed for use with standard real-time PCR platforms and supports consistent performance in molecular biology workflows.
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This kit in detail
- Gene Mutation Detection
- Real-time PCR
- Cambridge II Syndrome
- SERPINC1 Mutation
Cambridge II (SERPINC1) PCR Kit: Advanced Genetic Analysis
The imegen® Cambridge II kit is designed for the detection van de c.1246G>T (p.Ala416Ser) variant in the SERPINC1 gene using real-time PCR technology. This variant is associated with Cambridge II syndrome, a hereditary form of thrombophilia linked to altered antithrombin (AT) function.
Background
The SERPINC1 gene encodes antithrombin, a key regulator of the coagulation cascade. Antithrombin inhibits thrombin and other coagulation factors, helping to maintain the balance between clot formation and prevention.
The Cambridge II variant (p.Ala416Ser, also referred to as A368S) affects the functional activity of antithrombin. This alteration may influence its ability to regulate coagulation processes and is commonly studied in relation to thrombotic risk mechanisms.
Key Features
- Targeted Detection: Enables identification of the c.1246G>T (p.Ala416Ser) variant in the SERPINC1 gene.
- Real-time PCR Technology: Uses sequence-specific oligonucleotides and fluorescent hydrolysis probes for accurate and sensitive detection.
- Validated Components: Includes optimized master mixes to support consistent and reproducible assay performance.
- Platform Compatibility: Suitable for use with standard real-time PCR systems, including commonly used Thermo Fisher platforms.
Kit Components and Storage
- Cambridge II Master Mix: Contains specific oligonucleotides, labeled probes, and reaction components for target detection (store at -20°C).
- General Master Mix: Contains PCR enzymes, buffers, and stabilizers (store at 4°C).
Workflow and Application
The kit is designed for straightforward integration into standard laboratory workflows. It supports the analysis of genomic DNA, typically extracted from peripheral blood, and requires only a small input amount per reaction. The optimized protocol enables efficient and reproducible processing.
Conclusion
The imegen® Cambridge II kit provides a reliable solution for the analysis of genetic variation in the SERPINC1 gene. Its robust design and compatibility with standard laboratory equipment support consistent performance in molecular biology applications.
Master Mix: This component contains specific oligonucleotides, labeled probes, and water reagents optimized for PCR reactions targeting the SERPINC1 gene mutation.
General Master Mix: A conventional PCR enzyme mix containing 10-30% glycerol, <0.1% sodium azide, and buffers.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Cambridge II Syndrome qPCR kit.
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