p.D816V OncoKit
The p.D816V OncoKit detects and quantifies the p.D816V mutation in the KIT gene by digital PCR. More than 90 per cent of patients with systemic mastocytosis carry this mutation, which makes it central to the diagnosis of the disease. The assay is a two-target multiplex that quantifies the mutated and the wild-type allele in the same reaction. One kit covers 24 reactions. Research use only.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
- Digital PCR detection and quantification of KIT p.D816V
- Two-target multiplex: mutated and wild-type allele in one reaction
- FAM and VIC labelled TaqMan probes
- Heterozygous positive control of synthetic DNA included
- Validated against synthetic DNA with mutated and wild-type alleles
- 24 reactions per kit
The p.D816V OncoKit quantifies the p.D816V mutation in the KIT gene by digital PCR, for use in systemic mastocytosis.
Clinical background
Systemic mastocytosis is a rare haematological disorder in which mast cells proliferate abnormally and accumulate in tissues including the skin, the bone marrow and other extracutaneous sites, and it occurs in several subtypes. More than 90 per cent of patients carry the p.D816V mutation.
The mutation
The KIT gene encodes a protein involved in cell proliferation. The p.D816V substitution causes ligand-independent activation, which increases proliferation and reduces apoptosis. The mutation also confers resistance to certain tyrosine kinase inhibitors, so identifying it has consequences for treatment choice.
Method
The kit uses digital PCR with target-specific oligonucleotides and FAM and VIC labelled TaqMan probes, and quantifies the mutated and the wild-type allele in the same reaction. A heterozygous positive control of synthetic DNA containing both alleles is supplied, and the assay was validated against synthetic DNA with mutated and wild-type alleles.
Regulatory status
This kit is for research use only.
The kit is equipped with reagents for 24 reactions, including:
- Master Mix cKIT dPCR OncoKit: specific oligonucleotides, FAMTM and VICTM TaqMan probes, and
nuclease-free water to detect D816V of KIT gen by digital PCR. - Positive Control: mixture of synthetic DNA with the wild-type and mutated allele as
heterozygous positive control.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
MSDS p.D816V OncoKit
Frequently paired with this kit
Other assays and instruments lab teams add alongside the p.D816V OncoKit.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
