Huntington Disease Kit

Product Overview: Imegen® Huntington Disease Kit enables semi-quantitative analysis of CAG expansion in the HTT gene through HTT-C and HTT-L PCR assays, covering a 100-500 bp amplification range. It includes a positive control with normal alleles.

Key Features:

• Semi-Quantitative CAG Analysis
• Dual PCR Assays (HTT-C and HTT-L)
• Amplification Range: 100-500 bp
• Included Positive Control
• Compatible with Neurodegenerative Disease Assays
• DNA Requirement: 50 ng per Reaction
• Sample Type: Peripheral Blood
• 2 Reactions per Sample
• Targets: CAG and poly-CCG
• Quick Setup (45 mins)
• PCR Program Duration: 2h 15 mins
• Compatible with Standard Cyclers
• Sequencing with 6-Carboxyfluorescein (6-FAM) and NEDTM

About Huntington’s Disease: Huntington’s Disease is an inherited neurodegenerative disorder causing progressive brain neuron degeneration, impacting motor skills, cognition, and mental health. Diagnosis hinges on CAG triplet repeat analysis, with <26 repeats as normal, 27-35 intermediate, and >36 as pathological.

Imegen® Huntington Kit offers precise CAG expansion analysis with HTT-C (short-range) and HTT-L (long-range) PCR, covering polymorphic poly-CCG fragments. Sequencing via 6-FAM and NEDTM-labeled PCR products separates amplicons for size analysis.