Ashkenazim Son gDNA (EGFR)
Ashkenazim Son gDNA (EGFR) (SID-0004) is human genomic DNA from the GM24385 cell line, supplied as the wild-type background for EGFR mutation testing. The mutation status and the whole genome sequencing data of the line are publicly available through the Personal Genome Project. As a wild-type control it shows whether an EGFR assay stays negative where it should, and it serves as the background into which a mutation standard is diluted to a chosen allele frequency.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
- Human genomic DNA from the GM24385 cell line, catalogue SID-0004
- Wild-type background for EGFR mutation testing
- Mutation status and whole genome sequencing data public via the Personal Genome Project
- As a negative control and as a diluent for mutation standards
- Also suitable for validating sequencing and PCR protocols
Ashkenazim Son gDNA (EGFR) is the wild-type material in the EGFR series: genomic DNA from a published reference genome, without the EGFR variants that the mutation standards carry.
Two uses
As a negative control it establishes the false positive rate of an EGFR assay: any signal is by definition unwanted. As a diluent it sets the allele frequency, since a mutation standard diluted in wild-type DNA gives a series at a known mutant fraction.
Reference genome
The GM24385 line is part of the Personal Genome Project and its whole genome sequencing data and mutation status are freely accessible, so a result obtained with this DNA can be compared against a known dataset. The material is therefore also usable for validating whole genome and amplicon sequencing and PCR protocols and for calibrating fragmentation equipment.
The gDNA (human) wt contains Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8,0
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Ashkenazim Son gDNA (EGFR).
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
