Aspergillus Multiplex PCR Kit
The Aspergillus Multiplex PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the qualitative detection and differentiation of multiple Aspergillus species, including A. fumigatus, A. flavus, A. niger, and A. terreus, in human clinical samples. Based on advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly specific and reliable identification of clinically relevant fungal pathogens. Targeting conserved regions within the genomes of each species, the multiplex assay enables simultaneous detection and differentiation in a single reaction. An integrated internal control monitors DNA quality and detects potential PCR inhibition, ensuring confidence in diagnostic results. With optimized multiplex design and compatibility with widely used real-time PCR platforms, this kit is ideal for clinical diagnostics and research focused on invasive fungal infections, particularly in immunocompromised patients.
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This kit in detail
The Aspergillus Multiplex PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the qualitative detection and differentiation of multiple Aspergillus species, including A. fumigatus, A. flavus, A. niger, and A. terreus, in human clinical samples. Based on advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly specific and reliable identification of clinically relevant fungal pathogens.
Targeting conserved regions within the genomes of each species, the multiplex assay enables simultaneous detection and differentiation in a single reaction. An integrated internal control monitors DNA quality and detects potential PCR inhibition, ensuring confidence in diagnostic results.
With optimized multiplex design and compatibility with widely used real-time PCR platforms, this kit is ideal for clinical diagnostics and research focused on invasive fungal infections, particularly in immunocompromised patients.
- Multiplex detection of 4 Aspergillus species in one assay
- High sensitivity and specificity
- Multi-channel detection with internal control
- Ready-to-use, contamination-controlled workflow
Qualitative Multiplex PCR Detection of Aspergillus spp.
The Aspergillus Multiplex PCR Detection Kit is an in-vitro diagnostic real-time PCR assay developed for the qualitative identification and differentiation of Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, and Aspergillus terreus DNA in human samples. Utilizing hydrolysis probe-based multiplex detection, the assay delivers high sensitivity and specificity for multiple fungal targets in a single reaction.
The kit includes a ready-to-use master mix containing primers and probes for each target species, along with an internal control gene, enabling simultaneous validation of sample quality and amplification efficiency.
Molecular Background
Aspergillus species are opportunistic fungi responsible for a range of diseases collectively known as aspergillosis. These infections primarily affect immunocompromised individuals and can lead to severe respiratory conditions.
- Aspergillus fumigatus is the most common cause of invasive aspergillosis
- Aspergillus flavus is associated with respiratory infections and aflatoxin production
- Aspergillus niger is commonly found in the environment and can occasionally cause disease
- Aspergillus terreus is linked to invasive infections and may exhibit antifungal resistance
Early and accurate molecular detection is essential for timely diagnosis and appropriate antifungal treatment.
Assay Performance & Utility
The Aspergillus Multiplex PCR Detection Kit is designed for robust performance in routine diagnostic workflows. The assay uses multi-channel fluorescence detection to differentiate between species:
- FAM: A. fumigatus
- HEX: A. flavus
- ROX: A. niger
- Cy5.5: A. terreus
- Cy5: Internal Control
The assay incorporates “hot start” PCR technology to minimize non-specific amplification and enhance sensitivity. Uracil-DNA glycosylase (UDG) is included to prevent carry-over contamination, ensuring consistent and reproducible results.
With multiplex capability and high analytical performance, the kit enables efficient detection and differentiation of multiple fungal pathogens in a single assay, reducing time and laboratory workload.
Technical Specifications
- Assay Type: Multiplex Real-Time PCR (qPCR)
- Target: Aspergillus spp. DNA (A. fumigatus, A. flavus, A. niger, A. terreus)
- Detection Channels: FAM, HEX, ROX, Cy5.5 (targets), Cy5 (Internal Control)
- Sensitivity: High analytical sensitivity (validated in-house)
- Sample Types: Whole blood (EDTA) and other validated clinical samples
- Input Material: Extracted DNA
- Compatible Instruments: ABI 7500, QuantStudio 3/5/7, Bio-Rad CFX, Rotor-Gene Q, LightCycler 480, and equivalent systems
- Aspergillus qPCR Master Mix
- Aspergillus Positive Control
- Nuclease-free Water
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
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