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Blood RNA Extraction Kit – TRUPCR®
Blood RNA extraction is simplified and highly efficient with the TRUPCR® Blood RNA Extraction Kit, designed to purify high-quality cellular RNA from fresh, anticoagulated whole blood. Utilizing silica column technology and unique buffer systems, the kit ensures complete red cell lysis and selective RNA binding from white blood cells. The protocol eliminates the need for hazardous organic solvents like phenol or chloroform. Instead, RNA is safely and effectively isolated in under 1 hour with minimal hands-on time. The kit is compatible with key downstream molecular applications, including PCR, RT-PCR, cDNA synthesis, and NGS. With consistent yield, minimal RNase contamination, and compatibility with TRUPCR® BCR-ABL1, AML, and ALL panel kits, this solution is ideal for clinical and molecular laboratories requiring reliable RNA purification.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
- High-yield blood RNA extraction with minimal hands-on time
- No phenol, chloroform, or toxic reagents
- Removes PCR inhibitors like hemoglobin and heparin
- Compatible with TRUPCR® molecular panels (AML, ALL, BCR-ABL1, etc.)
What is in Blood RNA Extraction?
Blood RNA extraction refers to the process of isolating total RNA-especially from leukocytes in whole blood-for downstream molecular applications. Unlike DNA, RNA is more fragile and sensitive to degradation. Therefore, extraction methods must be fast, efficient, and gentle. The TRUPCR® kit enables the purification of high-quality RNA from whole blood using silica spin columns and proprietary buffers, offering an optimal workflow for RT-PCR and gene expression analysis.
Role of RNA Extraction in Molecular Diagnostics
RNA extracted from whole blood is essential for diagnosing hematological malignancies, monitoring minimal residual disease, and studying gene expression. It is particularly valuable in detecting fusion genes (e.g., BCR-ABL1) and in real-time PCR applications. The TRUPCR® Blood RNA Extraction Kit offers complete removal of inhibitors such as hemoglobin and heparin, which are common in blood-derived RNA, ensuring that the RNA is ready-to-use and yields reproducible results.
About the TRUPCR® Blood RNA Extraction Kit
This kit provides an advanced alternative to traditional RNA isolation methods like ultracentrifugation or phenol-chloroform extraction. It is designed for fresh samples (not frozen), and the protocol includes:
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Erythrocyte lysis
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Leukocyte enrichment
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RNase inactivation and lysis
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RNA binding and purification on a silica membrane
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Elution in 30–50 µl RNase-free water
Total RNA recovered is high-quality, with A260/A280 values exceeding 1.9, suitable for both qualitative and quantitative molecular workflows.
Principle and Workflow
The blood RNA extraction process involves:
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Selective lysis of red blood cells using proprietary buffer
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Recovery of white blood cells via centrifugation
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Lysis of leukocytes under denaturing conditions that degrade RNases
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RNA binding to the silica membrane
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Washing to remove proteins, salts, and contaminants
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Elution of purified RNA
This workflow ensures a fast and consistent preparation of RNA in less than one hour and is designed to prevent cross-contamination while enabling parallel processing of multiple samples.
Clinical Application
The TRUPCR® Blood RNA Extraction Kit supports a wide range of molecular diagnostics:
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Detection of fusion transcripts like BCR-ABL1 (chronic myeloid leukemia)
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Acute leukemia panels (e.g., AML, ALL)
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Monitoring treatment efficacy through gene expression profiling
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RT-PCR-based viral detection
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Use in clinical and translational research studies
Its reliability and simplicity make it ideal for use in routine diagnostics, clinical research labs, and high-throughput molecular screening programs.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Product Catalogus
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Blood RNA Extraction Kit – TRUPCR®.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
