CBFB-MYH11 Kit
The CBFB-MYH11 Kit identifies and quantifies the CBFB-MYH11 fusion gene, which results from the chromosomal rearrangement inv(16)(p13q22) and is used in the diagnosis and prognosis of acute myeloid leukaemia. The assay quantifies the type A variant, which accounts for 85 per cent of cases, alongside the endogenous reference gene ABL1. One kit covers 48 reactions for each of the two targets. Research use only.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
- Quantification of the CBFB-MYH11 type A fusion, inv(16)(p13q22)
- ABL1 as endogenous reference gene, quantified in parallel
- Reference plasmid for copy number calculation of both targets
- Limit of quantification 125 total copies for ABL1 and for the rearrangement
- Theoretical sensitivity MR4, 0.01 per cent, for minimal residual disease
- 10 µL of cDNA per reaction, from reverse transcription of total RNA
- 48 reactions per target
The CBFB-MYH11 Kit detects and quantifies the CBFB-MYH11 fusion gene by real-time PCR. The fusion results from the chromosomal rearrangement inv(16)(p13q22) and is used in the diagnosis and the prognosis of acute myeloid leukaemia.
Target
The assay amplifies and quantifies the type A variant of the fusion, which accounts for 85 per cent of cases, using target-specific oligonucleotides and hydrolysis probes.
Quantification
A reference plasmid is used to calculate the copy number of the rearrangement and of the endogenous gene ABL1, so the result can be expressed as a ratio. The limit of quantification is 125 total copies, both for ABL1 and for the rearrangement.
Minimal residual disease
The theoretical sensitivity is MR4, corresponding to 0.01 per cent, which is the level relevant to the follow-up of minimal residual disease.
Sample
Each reaction requires 10 µL of cDNA, obtained by reverse transcription of total RNA.
Regulatory status
This kit is for research use only.
The kit contents the necessary reagents to perform 48 reactions. The reagents included in the kit are the following:
- INV16 Master Mix: Specific oligonucleotides, labelled probes and water reagents for the PCR
reactions. - ABL1 Master Mix: Specific oligonucleotides, labelled probes and water reagents for the PCR
reactions. - INV16-ABL1 Standard: Positive control for INV16 rearrangement and ABL gene
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
MSDS NPM1 Mutation Kit
Frequently paired with this kit
Other assays and instruments lab teams add alongside the CBFB-MYH11 Kit.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
