Homologous Recombination Repair OncoKit
HRR OncoKit is a next-generation sequencing panel for genetic variants in the homologous recombination DNA repair pathway of solid tumours. Variants in this pathway determine eligibility for treatment with PARP inhibitors. The panel covers 38 genes including hotspot regions and fusion genes, detects copy number variations across the genome, and includes a 110-microsatellite panel for microsatellite instability testing. One kit covers 24 reactions. Research use only.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
- 38-gene panel for the homologous recombination repair pathway
- Hotspot regions and fusion genes included
- 110-microsatellite panel for microsatellite instability testing
- Copy number variation detection across the genome
- Molecular barcoding for low-input and degraded DNA
- 24 reactions per kit, with 24 unique 8-nucleotide indexes
HRR OncoKit analyses genetic variants in the homologous recombination DNA repair pathway of solid tumours by next-generation sequencing. Variants in this pathway are used to determine eligibility for PARP inhibitor treatment.
Panel content
The panel covers 38 genes selected for their role in solid tumours, and includes coverage of hotspot regions and of fusion genes. Sequencing multiple genomic regions in one run gives a picture of the tumour genotype that single-gene analysis cannot provide.
Microsatellite instability
A 110-microsatellite panel is included for microsatellite instability testing, which is used to identify cases that may respond to immunotherapy.
Copy number variations
Besides sequence variants, the panel detects copy number variations across the genome.
Sensitivity
Molecular barcoding is applied during library preparation, which supports detection in samples with low DNA input or degraded DNA.
Workflow
Library preparation uses Illumina adapters. The kit supplies 24 unique 8-nucleotide indexes in single-use strips, so 24 samples can be identified within one sequencing run.
Regulatory status
This kit is for research use only.
The kit contians the necessary reagents to perform 24 reactions. The reagents included in the kit are the following:
- Fragmentation Buffer (MSDS-D13): Buffer used for DNA fragmentation, prior to NGS libraries preparation.
- Fragmentation Enzyme (MSDS-D14): Enzyme used for DNA fragmentation, prior to NGS libraries preparation.
- Reagents Plate: Plate with all the reagents required to perform the DNA end repair, the Illumina adapters linkage, and amplifications done during the library preparation protocol.
- Beads and Buffers plate (MSDS-D15): Plate with magnetic particles and wash buffers, required for the DNA capture and its purification during the library preparation protocol.
- Index: Oligonucleotides with a unique 8 nucleotides identifier sequence compatible with the illumina adapters required to identify each sample during library preparation and NGS sequencing. The kit includes 24 different indexes, prepared in single-use strips.
- HRR Probes Strips: Biotinylated synthetic oligonucleotides complementary to the target regions of the target genes.
- Elution Buffer: Buffer used to elute the DNA.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
MSDS HHR OncoKit
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Homologous Recombination Repair OncoKit.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
