Chimerism Multiplex II Kit
The Imegen Quimera Screening Multiplex Plus II kit selects informative markers for the follow-up of patients after haematopoietic stem cell transplantation. It analyses 16 insertion and deletion polymorphisms in 8 multiplex real-time PCR reactions. CE marked for in vitro diagnostic use. The panel is complementary to Screening Multiplex Plus I: on its own it reaches a cumulative informativity of 99.4 per cent with the SRY and RhD markers, and 99.96 per cent when the markers of the other panel are analysed as well. One kit covers 10 genomic DNA samples, which is 5 recipient and donor cases.
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This kit in detail
- 16 insertion and deletion polymorphisms in 8 multiplex reactions
- Cumulative informativity 99.4 per cent, with SRY and RhD
- 99.96 per cent combined with Screening Multiplex Plus I
- Genomic DNA from peripheral blood or bone marrow, 450 ng each
- 7500 Fast, StepOne Plus or LightCycler 480
- 10 DNA samples or 5 cases per kit, REF IMG-116-74
The Imegen Quimera Screening Multiplex Plus II kit establishes which markers distinguish recipient from donor after an allogeneic stem cell transplant, so that one informative marker can be followed quantitatively afterwards.
Markers
The panel contains 16 insertion and deletion polymorphisms: 24I, 27D, 29D, 30D, 31I, 32I, 33I, 37I, 38I, 39I, 43I, 44I, 46I, 47I, 49I and 50I, alongside the SRY and RhD markers. The cumulative informativity of this panel is 99.4 per cent; analysing the markers of Screening Multiplex Plus I as well raises it to 99.96 per cent.
Method and sample
Detection is by real-time PCR with specific oligonucleotides and fluorescent hydrolysis probes. The limit of detection is 0.01 per cent on genomic DNA. The material required is genomic DNA from peripheral blood or bone marrow, 450 ng of the recipient sample taken before transplantation and 450 ng of the donor sample. The kit consists of one strip of 8 tubes with freeze-dried master mix. The assay runs on the 7500 Fast or StepOne Plus (Thermo Fisher) or on the LightCycler 480 (Roche), with follow-up in the Imegen Quimera software. The kit was developed with the Carlos Haya Regional Hospital in Malaga.
Regulatory status
This kit is CE marked for in vitro diagnostic use.
The kit contians the necessary reagents to perform 10 reactions. The reagents included in the kit are the following:
- Specific Master-Mix: Specific oligonucleotides and labelled probes
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
MSDS Chimerism Multiplex II Kit
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Chimerism Multiplex II Kit.
Add this kit to your quote
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