CLIA Jo-1
Chemiluminescence kit for the detection of Jo-1 antibodies in human serum or plasma.
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KleeYa® Automated CLIA Analyser
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This kit in detail
- Detection of anti-Jo-1 antibodies, IgG class
- Evaluating patients with signs and symptoms compatible with a connective tissue disease, especially muscle pain and limb weakness, concomitant pulmonary signs and symptoms, Raynaud phenomenon, and arthritis
- Testing for antibodies to Jo 1 is not useful in patients with a negative test for antinuclear antibodies
- Intended for human serum and plasma
- Recombinant Jo-1 antigen used as the antigen
Description
Jo-1 antigen is a histidyl-tRNA synthetase enzyme localized mainly in the cytoplasm. It is a member of the amino acyl-tRNA synthetase family of enzymes found in all nucleated cells. Anti-Jo-1 antibodies are highly specific for myositis. They are associated with polymyositis (20-40%). Less frequently, these antibodies are also found in dermatomyositis and myositis related to other connective tissue systemic diseases.
Anti-Jo-1 antibodies occur in up to 50% of patients with interstitial pulmonary fibrosis and symmetrical polyarthritis. More than 70% of patients positive for Jo-1 antibodies have interstitial lung disease. The anti-Jo-1 antibodies level corresponds with the disease activity.
The antisynthetase syndrome comprises presence of anti-Jo-1 antibodies, myositis, interstitial lung disease, rheumatoid arthritis, or Raynaud’s phenomenon. The disease manifestation differs depending on the type of anti-synthetase antibody (most often anti-Jo-1 antibody).
Antigen Used: Recombinant Jo-1 antigen.
Technical data
- Assay stability: 30 days on board stability / In use stability until the expiration date at storage temperature 2-8 °C
- Sample matrix: Serum, Plasma
- Sample volume: 10 μL
- Measuring range: 0,5-320 U/ml
- Complementary products: Anchor® Tips, Stackable Cuvette, KleeYa Trigger Pack, KleeYa Wash buffer
- Note: The kits are CE-IVD certified and intended for professional use.
Specifications
- Catalog Number: CL-Jo1050
- Size: 50 tests
- Regulatory status: CE IVD
- Clinical topic: Immunology & Autoimmune Diseases
- Diagnostic panel: Rheumatic Diseases
- Producer: TestLine Clinical Diagnostics s.r.o.
References
- 19393194,20854935,20933614,20175839,20833272,20538173,20146695,22743034,15804705
- Targoff IN. Autoantibodies in polymyositis. Rheum Dis Clin North Am. 1992 May;18(2):455-82. PMID: 1378227.
- Zampieri S, Ghirardello A, Iaccarino L, Tarricone E, Gambari PF, Doria A. Anti-Jo-1 antibodies. Autoimmunity. 2005 Feb;38(1):73-8. doi: 10.1080/08916930400022640. PMID: 15804708.
- Mileti LM, Strek ME, Niewold TB, Curran JJ, Sweiss NJ. Clinical characteristics of patients with anti-Jo-1 antibodies: a single center experience. J Clin Rheumatol. 2009 Aug;15(5):254-5. doi: 10.1097/RHU.0b013e3181b0e910. PMID: 19590436; PMCID: PMC3380442.
- Jiang M, Dong X, Zheng Y. Clinical characteristics of interstitial lung diseases positive to different anti-synthetase antibodies. Medicine (Baltimore). 2021 May 14;100(19):e25816. doi: 10.1097/MD.0000000000025816. PMID: 34106621; PMCID: PMC8133147.
- de Andrade VP, De Souza FHC, Behrens Pinto GL, Shinjo SK. The relevance of anti-Jo-1 autoantibodies in patients with definite dermatomyositis. Adv Rheumatol. 2021 Feb 19;61(1):12. doi: 10.1186/s42358-021-00171-x. PMID: 33608062.
- Mozaffar T, Pestronk A. Myopathy with anti-Jo-1 antibodies: pathology in perimysium and neighbouring muscle fibres. J Neurol Neurosurg Psychiatry. 2000 Apr;68(4):472-8. doi: 10.1136/jnnp.68.4.472. PMID: 10727483; PMCID: PMC1736872.
- Witt LJ, Curran JJ, Strek ME. The Diagnosis and Treatment of Antisynthetase Syndrome. Clin Pulm Med. 2016 Sep;23(5):218-226. doi: 10.1097/CPM.0000000000000171. PMID: 27594777; PMCID: PMC5006392.
- Reagent Cartridge with specific reagents for the assay
- magnetic particles
- calibrators
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Safety information
Product brochure
Declaration of conformity
Frequently paired with this kit
Other assays and instruments lab teams add alongside the CLIA Jo-1.
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