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Echinococcus multilocularis (Em18) IgG ELISA Kit
Echinococcus multilocularis (Em18) IgG ELISA Kit Alveolar echinococcosis is caused by an infection with the cestode Echinococcus multilocularis and is a very dangerous disease for humans. Adult worms mature in the intestine of the definitive host (usually fox, occasionally dog or cat) and the eggs are released in the feces. Accidental ingestion of soil, berries or vegetables contaminated with eggs iniate the infection in humans. Oncospheres hatch in the duodenum, penetrate the intestine and are carried via the bloodstream to organs. Although alveolar cysts have been found in other tissues, the most common site of development is the liver. The cysts are not limited by a membrane but penetrate the tissues and may resemble a slow growing carcinoma. Surgical removing is difficult but drugs have been found that stop the development of the metacestode. The Em18-ELISA is suitable for monitoring serologically the treatment efficacy in AE patients. Extensive evaluations revealed that anti-recEm18 serum antibody levels rapidly declined to undetectable concentrations upon complete surgical resection of the parasite lesion, usually within a time span of several months to less than one year. Inactivation of the parasite by drug treatment results in a full calcification of the lesion(s), plus negativation of both Em18-serology and PET-scan (see refs. below).
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Echinococcus multilocularis (Em18) IgG ELISA Kit
Alveolar echinococcosis is caused by an infection with the cestode Echinococcus multilocularis and is a very dangerous disease for humans. Adult worms mature in the intestine of the definitive host (usually fox, occasionally dog or cat) and the eggs are released in the feces. Accidental ingestion of soil, berries or vegetables contaminated with eggs iniate the infection in humans. Oncospheres hatch in the duodenum, penetrate the intestine and are carried via the bloodstream to organs. Although alveolar cysts have been found in other tissues, the most common site of development is the liver. The cysts are not limited by a membrane but penetrate the tissues and may resemble a slow growing carcinoma. Surgical removing is difficult but drugs have been found that stop the development of the metacestode. The Em18-ELISA is suitable for monitoring serologically the treatment efficacy in AE patients. Extensive evaluations revealed that anti-recEm18 serum antibody levels rapidly declined to undetectable concentrations upon complete surgical resection of the parasite lesion, usually within a time span of several months to less than one year. Inactivation of the parasite by drug treatment results in a full calcification of the lesion(s), plus negativation of both Em18-serology and PET-scan (see refs. below).
- High sensitivity detection of E. multilocularis
- 8-wells polystyrene breakable strips
- IVDR certified
- Detection of antibodies in the serum of humans
ELISA kit for human alveolar echinococcosis patient follow-up
96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with recombinant antigen (Em18) from Echinococcus multilocularis.
8-well polystyrene breakable strips : useful and economic format for laboratories with a small workload.
CE registration: CH-201708-0010
Paired pre- and post-surgical serum samples of 12 patients with confirmed alveolar echinococcosis and having had a radical or non-radical surgery were studied. Pre-surgically, 9 patients (75%) had an index >1. Among these patients, 5 had negative post-surgical results. But in all 12 patients, post-surgical Em18 antibody levels dropped and were significantly lower than in pre-surgical samples.
Serum samples of 25 patients with confirmed alveolar echinococcosis without surgery but with stable disease under antiparasitic chemotherapy were studied. 18 (72%) of them had an index >1 (median index 6.3).
Serum samples of 7 patients with confirmed alveolar echinococcosis without surgery but with progressive disease under antiparasitic chemotherapy were studied. 6 (86%) of them had an index >1 (median index 13.8).
96 wells coated with Echinococcus multilocularis Em18 antigen.
Dilution buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A – AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
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