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Fasciolosis is one of the most frequently encountered autochthonous helminthic infection in Central Europe. The immunodiagnosis of Fasciola hepatica infections is challenged by high serological cross-reactivity with other (hepatic) parasitological infections encountered in Central Europe, such as alveolar echinococcosis, toxocarosis and ascariosis, but also other parasitic infections acquired during overseas travel. The SAP-2 recombinant antigen shows a high specificity, especially with patients with other parasitic infections. It is a suitable serological test for routine diagnosis of human fasciolosis, particularly if the results are supported by clinical history.
96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with a recombinant antigen from F. hepatica.
8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload.
CE registration: CH-201504-0006
The test detects Fasciola hepatica infections.
A sensitivity of 77% was found with 13 sera from patients with parasitologically proven Fasciola hepatica infections.
A specificity of 99% was found with 99 sera of blood donors (Swiss).
A specificity of 98 % was found with 100 sera from patients of an infectiology unit (Swiss).
A specificity of 97% was found with patients with other parasitc infections.
96 wells coated with a recombinant antigen from Fasciola hepatica.
Dilution buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A – AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
Instructions for Use
References
Figueroa-Santiago, O., Delgado, B. and Espino, A.M. (2011) Fasciola hepatica saposin-like protein-2-based ELISA for the serodiagnosis of chronic human fascioliais. Diagnostic Microbiology and Infectious Disease 70, 355-361.
Gottstein B, Schneeberger M, Boubaker G, Merkle B, Huber C, et al. (2014) Comparative Assessment of ELISAs Using Recombinant Saposin-Like Protein 2 and recombinant Cathepsin L-1 from Fasciola hepatica for the Serodiagnosis of Human Fasciolosis. PLoS Negl Trop Dis 8(6): e2860. doi:10.1371/journal.pntd.0002860