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Trichinellosis is caused by Trichinella spiralis, a parasitic nematode of pork, horse and wild animals. Humans can be infected by eating raw or undercooked meat from infected animals. Most infected people do not show any symptoms. However, in some cases, symptoms appear during intestinal stage (diarrhea and abdominal pain<9 and muscular stage (muscle pain, fever, edema). Diagnosis is based signs and symptoms, but, in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. Our ELISA kit have shown appropriate performances for the serological screening of trichinellosis.
96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with E/S antigens from cultivated larvae of Trichinella spiralis.
8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload.
CE registration: CH-202002-0011
The test detects Trichinella spiralis infections.
A sensitivity of 95% was found with 55 sera from patients suffering from trichinellosis.
A specificity of 98% was found with 149 sera of blood donors (Swiss).
A specificity of 98% was found with 100 sera from patients of an infectiology unit (Swiss).
A specificity of 90% was found with 62 sera from trichinellosis suspected patients but where this disease had been certainly ruled out.
A specificity of 93% was found with 45 sera of patients with other parasitic infections.
96 wells coated with E/S antigens from cultivated larvae of Trichinella spiralis.
Dilution buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A – AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
Instructions for Use
References
Gomez-Morales, M.A., Ludovisi, A., Amati, M., Cherchi, S., Pezzotti, P. and Pozio, E. (2008) Validation of an Enzyme-Linked Immunosorbent Assay for diagnosis of human trichinellosis. Clin. Vaccine. Immunol. 15: 1723–1729.
Yong Yang, Ya Nan Cai, Ming Wei Tong, Na Sun, Yin Hua Xuan, Yan Jun Kang, Vallée, I., Boireau, P., Shi Peng Cheng and Ming Yuan Liu. (2016) Serological tools for detection of Trichinella infection in animals and humans. One Health 2: 25-30.