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Acanthocheilonema viteae IgG ELISA Kit
Acanthocheilonema viteae IgG ELISA Kit Human filariasis are caused by a variety of nematodes (Loa Loa, Onchocerca volvulus, Dracunculus medinensis, Wuchereria bancrofti, Brugia malayi). The diagnosis is based on clinical evidences, an history of living in an endemic area, hypereosinophilia and the detection of microfilariae. The finding of an adult filaria is a proof of infection but is frequently unsuccessful. Serological methods using indirect immunofluorescence assays on frozen sections of adult worms of animal origin (Acanthocheilonema viteae, Dirofilaria immitis, Setaria sp.) and immuno-electrophoresis can both help for diagnosis. These methods are often positive in cases of occult filariasis or when microfilaremia is negative, they are less sensitive in patients with detectable microfilariae. Our ELISA assay uses also the antigenic homologies observed between human and animal filariae. The ELISA procedure is more reproducible and less tedious than IFA on worm sections.
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Acanthocheilonema viteae IgG ELISA Kit
Human filariasis are caused by a variety of nematodes (Loa Loa, Onchocerca volvulus, Dracunculus medinensis, Wuchereria bancrofti, Brugia malayi). The diagnosis is based on clinical evidences, an history of living in an endemic area, hypereosinophilia and the detection of microfilariae. The finding of an adult filaria is a proof of infection but is frequently unsuccessful. Serological methods using indirect immunofluorescence assays on frozen sections of adult worms of animal origin (Acanthocheilonema viteae, Dirofilaria immitis, Setaria sp.) and immuno-electrophoresis can both help for diagnosis. These methods are often positive in cases of occult filariasis or when microfilaremia is negative, they are less sensitive in patients with detectable microfilariae. Our ELISA assay uses also the antigenic homologies observed between human and animal filariae. The ELISA procedure is more reproducible and less tedious than IFA on worm sections.
- High sensitivity detection of A. viteae
- 8-wells polystyrene breakable strips
- IVDR certified
- Detection of antibodies in the serum of humans
ELISA kit for the diagnosis of human filariasis
96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with somatic antigens from adult females of Acanthocheilonema viteae.
8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload.
CE registration: H-CH/CA01/IVD/01755
Sensitivity of 95% with sera of suspected and confirmed cases of filariasis.
Specificity of 98% with sera of blood donors (from Switzerland).
Crossreactivities are found in cases of trichinellosis, hydatidosis, fascioliasis, ankylostomiasis and strongyloidiasis (40 to 83%).
96 wells coated with somatic antigens from adult females of Acanthocheilonema viteae.
Dilution buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A – AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
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