Factor II kit
Factor II PCR Kit The Factor II PCR Kit is a molecular biology tool developed for the analysis of the F2 gene, which is involved in blood coagulation. It enables the detection of specific genetic variants using a validated real-time PCR assay with fluorescent hydrolysis probes. The kit is designed to deliver accurate and reproducible results while requiring only 50 ng of genomic DNA per reaction. Its optimized format supports consistent performance and straightforward integration into standard laboratory workflows. This kit provides a reliable solution for the study of genetic variation in the F2 gene within molecular biology applications. Note that this kit is for Research Use Only (RUO)
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This kit in detail
Factor II PCR Kit
The Factor II PCR Kit is a molecular biology tool developed for the analysis of the F2 gene, which is involved in blood coagulation. It enables the detection of specific genetic variants using a validated real-time PCR assay with fluorescent hydrolysis probes.
The kit is designed to deliver accurate and reproducible results while requiring only 50 ng of genomic DNA per reaction. Its optimized format supports consistent performance and straightforward integration into standard laboratory workflows.
This kit provides a reliable solution for the study of genetic variation in the F2 gene within molecular biology applications.
Note that this kit is for Research Use Only (RUO)
- Real-Time PCR
- F2 Gene Focus
- Dual Probes
- Target Multiplexing
Factor II PCR Kit: Advanced Genetic Analysis
The Factor II PCR Kit is designed for the analysis of genetic variation within the F2 gene, which plays a role in blood coagulation. It enables the detection of the c.*97G>A variant and the corresponding wild-type sequence in the 3′ UTR region of the prothrombin (F2) gene using real-time PCR technology.
Key Features
- Targeted Detection: Allows identification of the c.*97G>A variant and wild-type allele within the F2 gene.
- Real-time PCR Technology: Uses sequence-specific oligonucleotides and fluorescent hydrolysis probes for accurate and sensitive detection.
- Positive Control: Includes a heterozygous genomic DNA control to support assay performance and result verification.
- Validated Performance: Tested with reference and synthetic DNA to ensure consistent and reproducible results.
- Sample Input: Requires 50 ng of genomic DNA, typically extracted from peripheral blood.
- Platform Compatibility: Suitable for use with standard real-time PCR instruments and fluorescence detection channels.
Workflow and Application
The kit is designed for straightforward integration into standard laboratory workflows. Its optimized components and clear protocol support efficient processing and consistent performance across different laboratory settings.
Conclusion
The Factor II PCR Kit provides a reliable solution for the analysis of genetic variants in the F2 gene. With its robust design and compatibility with commonly used laboratory equipment, it supports high-quality molecular biology applications.
The kit contents the necessary reagents to perform 48 reactions. The reagents included in the kit are the following:
- Factor II Master Mix: Specific oligonucleotides, labelled probes and water reagents for the
PCR reactions. - General Master Mix: Conventional PCR enzyme containing 10-30% glycerol and buffers.
- Positive Control: Heterozygous positive control for the c.20210G>A mutation in F2 gene.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
MSDSHLA Factor II kit
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Factor II kit.
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