Factor II kit

Factor II PCR Kit: Advanced Genetic Analysis

The Factor II PCR Kit is designed for the analysis of genetic variation within the F2 gene, which plays a role in blood coagulation. It enables the detection of the c.*97G>A variant and the corresponding wild-type sequence in the 3′ UTR region of the prothrombin (F2) gene using real-time PCR technology.

Key Features

• Targeted Detection: Allows identification of the c.*97G>A variant and wild-type allele within the F2 gene.
• Real-time PCR Technology: Uses sequence-specific oligonucleotides and fluorescent hydrolysis probes for accurate and sensitive detection.
• Positive Control: Includes a heterozygous genomic DNA control to support assay performance and result verification.
• Validated Performance: Tested with reference and synthetic DNA to ensure consistent and reproducible results.
• Sample Input: Requires 50 ng of genomic DNA, typically extracted from peripheral blood.
• Platform Compatibility: Suitable for use with standard real-time PCR instruments and fluorescence detection channels.

Workflow and Application

The kit is designed for straightforward integration into standard laboratory workflows. Its optimized components and clear protocol support efficient processing and consistent performance across different laboratory settings.

Conclusion

The Factor II PCR Kit provides a reliable solution for the analysis of genetic variants in the F2 gene. With its robust design and compatibility with commonly used laboratory equipment, it supports high-quality molecular biology applications.